Tracing back ancient oral microbiomes and oral pathogens using dental pulps from ancient teeth.
Tracing back ancient oral microbiomes and oral pathogens using dental pulps from ancient teeth.
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DOI：
10.1038/s41522-016-0008-8
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发表时间：
2016
影响因子：
9.2
通讯作者：
Desnues C
中科院分区：
文献类型：
--
作者：
Rascovan N;Huynh H;Chouin G;Adekola K;Georges-Zimmermann P;Signoli M;Desfosses Y;Aboudharam G;Drancourt M;Desnues C
古代牙髓是非常珍贵的样本，因为它们保存了人类和血液传播病原体的DNA。然而，人们对牙髓中存在的微生物群落知之甚少。在这里，我们分析了来自不同时期和地理区域的古代和现代牙髓样本，发现它们由不同的微生物群落定殖，这些微生物群落可以与其他口腔样本区分开来。我们发现，尽管存在环境细菌，但古老的牙髓保存了清晰且保存良好的口腔微生物记录。我们能够在古代和现代牙髓中检测到几种不同的口腔病原体，这些病原体通常与牙周疾病有关。因此，我们表明，古代牙髓不仅是人类和全身感染DNA的宝贵来源，而且也是研究古代口腔微生物组的一个开放窗口。
Ancient dental pulps are highly precious samples because they conserve DNA from humans and blood-borne pathogens for ages. However, little is known about the microbial communities present in dental pulps. Here, we analyzed ancient and modern dental pulp samples from different time periods and geographic regions and found that they are colonized by distinct microbial communities, which can be differentiated from other oral cavity samples. We found that despite the presence of environmental bacteria, ancient dental pulps conserve a clear and well-conserved record of oral microbes. We were able to detect several different oral pathogens in ancient and modern dental pulps, which are commonly associated with periodontal diseases. We thus showed that ancient dental pulps are not only valuable sources of DNA from humans and systemic infections, but also an open window for the study of ancient oral microbiomes.