Progesterone binding in the mouse and rat uterus.
Progesterone binding in the mouse and rat uterus.
复制标题
黄体酮在小鼠和大鼠子宫中的结合。
DOI：
10.1210/endo-91-3-738
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发表时间：
1972
期刊：
影响因子：
4.8
通讯作者：
B. O’Malley
中科院分区：
文献类型：
--
作者：
P. Feil;S. Glasser;D. Toft;B. O’Malley
用蔗糖-甘油梯度分析法检测了小鼠和大鼠子宫胞液中的孕激素结合成分。小鼠子宫胞液中的结合组分的沉降值为4S和6.5S，大鼠的沉降值为4S和7S。在这两个物种中的结合成分被发现是特定的子宫和蛋白质的性质。结合量取决于动物的内分泌状态。去势破坏了结合成分，但它们可以在雌激素刺激后恢复到完整的水平。竞争研究表明，孕酮的特异性很高，孕酮的结合很少是由于皮质类固醇结合球蛋白（CBG）的污染。平衡测量表明受体-激素相互作用具有高亲和力（Kd 10 - 8 - 10 - 9M），而动力学测量表明受体-激素复合物的解离非常迅速，甘油可使其稳定。t...
Progesterone—binding components in the uterine cytosol from the mouse and rat have been detected using sucrose—glycerol gradient analysis. The binding components in mouse uterine cytosol had sedimentation values of 4S and 6.5S and for the rat, values of 4S and 7S were determined. The binding components in both species were found to be specific for the uterus and to be protein in nature. The amount of binding was dependent upon the endocrine status of the animal. Castration destroyed the binding components, but they could be restored to intact levels following estrogen stimulation. Competition studies showed a high specificity for progesterone and that little of the progesterone binding was due to contamination by corticosteroid binding globulin (CBG). Equilibrium measurements have indicated a high affinity of the receptor—hormone interaction (Kd 10-8 – 10-9M) while kinetic measurements have revealed a very rapid dissociation of the receptor—hormone complex which could be stabilized somewhat by glycerol. T...