Synthetic two-way communication between mammalian cells
Synthetic two-way communication between mammalian cells
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DOI：
10.1038/nbt.2351
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发表时间：
2012-10-01
影响因子：
46.9
通讯作者：
Fussenegger, Martin
中科院分区：
文献类型：
--
作者：
Bacchus, William;Lang, Moritz;Fussenegger, Martin
合成生物学启发的控制设备的设计使整个哺乳动物细胞能够接收，处理和传递代谢信息，并通过合成多通道网络相互通信，这可能为多细胞生物体的组织和未来的临床干预提供新的见解。在这里，我们描述的通信网络，协调行为在个别哺乳动物细胞响应细胞间的代谢信号。我们设计了发送器、处理器和接收器细胞，它们以类似于自然细胞间通信网络的方式相互作用，例如多步信息处理级联、基于前馈的信号循环和双向通信。模拟脊椎动物四肢（2）和脉管系统（3-5）发育中的自然控制系统的工程化双向通信装置用于编程血管内皮细胞层中的时间渗透性。这些合成的多细胞通信系统可能会激发未来的治疗或组织工程策略。
The design of synthetic biology-inspired control devices enabling entire mammalian cells to receive, process and transfer metabolic information and so communicate with each other via synthetic multichannel networks may provide new insight into the organization of multicellular organisms and future clinical interventions(1). Here we describe communication networks that orchestrate behavior in individual mammalian cells in response to cell-to-cell metabolic signals. We engineered sender, processor and receiver cells that interact with each other in ways that resemble natural intercellular communication networks such as multistep information processing cascades, feed-forward-based signaling loops, and two-way communication. The engineered two-way communication devices mimicking natural control systems in the development of vertebrate extremities(2) and vasculature(3-5) was used to program temporal permeability in vascular endothelial cell layers. These synthetic multicellular communication systems may inspire future therapies or tissue engineering strategies.