Tissue factor is a major stimulus for vegetation formation in enterococcal endocarditis in rabbits.
Tissue factor is a major stimulus for vegetation formation in enterococcal endocarditis in rabbits.
复制标题
组织因子是兔肠球菌性心内膜炎赘生物形成的主要刺激因素。
DOI：
10.1172/jci111383
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Sande,MA
中科院分区：
文献类型：
--
作者：
Drake,TA;Rodgers,GM;Sande,MA
我们使用兔模型研究了肠球菌性心内膜炎赘生物形成中局部启动凝血的可能机制。使用开发用于培养细胞的测定法评估新鲜切除的主动脉瓣的接触活化和组织因子表达。单独的细菌缺乏促凝血活性，未发生切除瓣膜对血浆的接触活化。4天感染但非对照瓣膜表达显著的组织因子活性（231 +/- 17 mU vs. 51 +/- 7 SE），这与赘生物中的细菌数量无关。组织因子活性也存在于瓣膜感染1和2天，以及从粒细胞和单核细胞减少症的动物。我们的研究结果表明，组织因子，表达宿主细胞在感染，是一个主要的刺激纤维蛋白沉积在植被发展。
We examined the possible mechanisms of local initiation of coagulation in vegetation formation in enterococcal endocarditis by using a rabbit model. Contact activation and tissue factor expression by freshly excised aortic valves were assessed using assays developed for use with cultured cells. Bacteria alone lacked procoagulant activity and contact activation of plasma by excised valves did not occur. 4-d infected but not control valves expressed significant tissue factor activity (231 +/- 17 mU vs. 51 +/- 7 SE), which did not correlate with numbers of bacteria in vegetations. Tissue factor activity was also present in valves from rabbits infected for 1 and 2 d, as well as those from granulocytopenic and monocytopenic animals. Our findings suggest that tissue factor, expressed by host cells in response to infection, is a major stimulus for fibrin deposition in vegetation development.