Identification of new self-incompatibility alleles in sweet cherry (Prunus avium L.) and clarification of incompatibility groups by PCR and sequencing analysis
Identification of new self-incompatibility alleles in sweet cherry (Prunus avium L.) and clarification of incompatibility groups by PCR and sequencing analysis
复制标题
通过 PCR 和测序分析鉴定甜樱桃 (Prunus avium L.) 新的自交不亲和性等位基因并澄清不亲和性群体
DOI：
10.1007/s001220051700
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发表时间：
2001
影响因子：
5.4
通讯作者：
F. Kappel
中科院分区：
文献类型：
--
作者：
P. Wiersma;Z. Wu;L. Zhou;C. Hampson;F. Kappel
摘要对甜樱桃品种进行正确的交叉亲和性组合分配对樱桃果实的高效生产具有重要意义。尽管在文献中有相当大的混乱，这些群体仍然是预测育种者和种植者授粉效果的有效工具。从樱桃S-RNase序列中得到的PCR片段与特定的s等位基因表型一致。25个基因组DNA片段，代表6个最常见的等位基因，被克隆和测序。此外，还发现了4个新的s等位基因片段。这些基因组序列和cDNA序列在具有相同s等位基因的栽培品种之间是不变的。利用序列数据，建立了基于PCR和限制性内切酶的s基因型快速分析方法。讨论了s等位基因测定的片段模式分析和描述。该方法被用于表征70个北美甜樱桃品种的s等位基因组成，包括许多来自加拿大农业和农业食品部（位于不列颠哥伦比亚省Summerland和安大略省Vineland）的加拿大育种计划的命名品种。发现了已发表的s -等位基因分配与PCR数据之间的一些差异，并提出了一个新的品种s -等位基因分配清单。
Abstract Correct assignment of sweet cherry cultivars to cross-compatibility groups is important for the efficient production of cherry fruit. Despite considerable confusion in the literature, these groups continue to be an effective tool for predicting pollination effectiveness for breeders and growers. PCR fragments generated from cherry S-RNase sequences coincided with specific S-allele phenotypes. Twenty five genomic DNA fragments, representing the six most common alleles, were cloned and sequenced. In addition, fragments were characterized from four new S-alleles. These genomic and cDNA sequences were invariant among cultivars with the same S-allele. Using the sequence data, PCR and restriction enzyme-based methodology was developed for rapid analysis of S-genotypes. Analysis and description of fragmentation patterns for S-allele determination are discussed. The method was utilized to characterize the S-allele composition of 70 sweet cherry cultivars obtained from collections in North America, including many of the named releases from the Canadian breeding programs at Agriculture and Agri-Food Canada in Summerland, B.C., and Vineland, Ontario. A number of differences between published S-allele assignments and PCR data were discovered and a new listing of cultivar S-allele assignments is presented.