Effects of halothane on transport of 5-hydroxytryptamine by platelet membranes.
Effects of halothane on transport of 5-hydroxytryptamine by platelet membranes.
复制标题
氟烷对血小板膜转运 5-羟色胺的影响。
DOI：
10.1042/bj2350033
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
D. Rannels
中科院分区：
文献类型：
--
作者：
C. A. Watkins;L. Mylin;D. Rannels
测定了牛血小板质膜小泡对Na+依赖的5-羟色胺(5-羟色胺)的摄取和血小板裂解产物对储存小泡的ATP依赖的5-HT摄取。Na+依赖的摄取是温度依赖的，被丙咪胺抑制，符合Michaelis-Menten动力学(表观Km，0.12+/-0.02微米；Vmax)。每毫克蛋白质559+/-54pmol/min。氟烷对5-羟色胺在质膜囊泡中的钠依赖转运无影响。依赖于ATP的5-羟色胺向储存颗粒的转运也符合Michaelis-Menten动力学(表观Km0.34+/-0.03微米；Vmax)。34.3+/-1.7pmol/min每毫克蛋白质)，并可被去甲肾上腺素(去甲肾上腺素)抑制，但不被丙咪嗪抑制。暴露在氟烷中的颗粒导致Vmax进行性下降。结果表明麻醉剂可能破坏了血小板的功能。
Na+-dependent uptake of 5-HT (5-hydroxytryptamine) into plasma membrane vesicles derived from bovine blood platelets and ATP-dependent 5-HT uptake into storage vesicles in platelet lysates were measured. Na+-dependent uptake was temperature-dependent, inhibited by imipramine and exhibited Michaelis-Menten kinetics (apparent Km, 0.12 +/- 0.02 microM; Vmax. 559 +/- 54 pmol/min per mg of protein. Halothane had no effect on Na+-dependent transport of 5-HT in plasma-membrane vesicles. ATP-dependent 5-HT transport into storage granules also exhibited Michaelis-Menten kinetics (apparent Km 0.34 +/- 0.03 microM; Vmax. 34.3 +/- 1.7 pmol/min per mg of protein) and was inhibited by noradrenaline (norepinephrine), but not by imipramine. Exposure of the granules to halothane resulted in a progressive decrease in Vmax. The results demonstrate a possible site for disruption of platelet function by anaesthetics.