Efficient osteoblast differentiation from mouse bone marrow stromal cells with polylysin-modified adenovirus vectors
Efficient osteoblast differentiation from mouse bone marrow stromal cells with polylysin-modified adenovirus vectors
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DOI：
10.1016/j.bbrc.2008.12.055
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发表时间：
2009-01-30
影响因子：
3.1
通讯作者：
Mizuguchi, Hiroyuki
中科院分区：
文献类型：
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作者：
Tashiro, Katsuhisa;Kondo, Asami;Mizuguchi, Hiroyuki
骨髓基质细胞（BMSCs）有望成为组织再生的来源，因为它们可以分化为多种细胞类型。建立高效的骨髓间充质干细胞基因转移系统是其应用于再生医学的关键。在这项研究中，我们比较了使用纤维修饰的腺病毒（Ad）载体在小鼠原代BMSCs中的转导效率，并证明了AdK 7，其在纤维结的C-末端携带聚赖氨酸（K7）肽，可以有效地在BMSCs中表达转基因。值得注意的是，AdK 7以3，000个载体颗粒/细胞有力地驱动了超过90%的BMSC的反式基因表达。此外，我们发现，在体外和体内的成骨潜能的骨髓基质细胞显着促进Runx 2基因转导使用AdK 7。这些结果表明，这种转导系统可能成为基于油BMSC的治疗应用的有力工具。(C)2008年爱思唯尔公司All rights reserved.
Bone marrow stromal cells (BMSCs) are expected to be a source for tissue regeneration because they call differentiate into Multiple cell types. Establishment of efficient gene transfer systems for BMSCs is essential for their application to regenerative medicine. In this Study, we compared the transduction efficiency in mouse primary BMSCs by using fiber-modified adenovirus (Ad) vectors, and demonstrated that AdK7, which harbors a polylysin (K7) peptide in the C-terminus of the fiber knob, could efficiently express a transgene in BMSCs. Notably, AdK7 robustly drove trans,gene expression it) more than 90% of the BMSCs at 3,000 Vector particles/cell. Furthermore, we showed that in vitro and in vivo osteogenic potential of BMSCs was dramatically promoted by the transduction of Runx2 gene using AdK7. These results indicate that this transduction system could be a powerful tool for therapeutic applications based oil BMSCs. (C) 2008 Elsevier Inc. All rights reserved.