Molecular characterization and expression analysis of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE gene family in Paeonia suffruticosa
Molecular characterization and expression analysis of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE gene family in Paeonia suffruticosa
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DOI：
10.1007/s00299-020-02573-5
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发表时间：
2020-07
影响因子：
6.2
通讯作者：
Shunli Wang;Xiuxia Ren;Jingqi Xue;Yu-qian Xue;Xiaodan Cheng;Xiaogai Hou;Xiuxin Zhang
中科院分区：
文献类型：
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作者：
Shunli Wang;Xiuxia Ren;Jingqi Xue;Yu-qian Xue;Xiaodan Cheng;Xiaogai Hou;Xiuxin Zhang
关键信息在牡丹中共鉴定出16个PsSPL基因，PsSPL基因主要调控开花时间、侧芽和种子的发育，以及幼苗向成体的转化。本文报道了牡丹（Peaonia suffruticosaAndr.）和9个具有miR 156靶位点的PsSPL。系统发育分析。Ssuffruticosa和Psopsis可分为6个类群，PsSPL与Psopsis的SPL在同一类群中高度相关，启动子区顺式元件分析表明PsSPL基因在生理过程和发育事件中发挥作用。表达分析表明，大多数PsSPL基因表现出高表达水平的组织和器官在这里检查。PsSPL 1、PsSPL 2、PsSPL 8、PsSPL 9、PsSPL 12和PsSPL 16的表达水平随时间的推移而增加，而PsSPL 1A和PsSPL 1Bin的表达水平随时间的推移而降低，这表明它们可能受到幼胚到成体阶段转换的调节。此外，PsSPL 2、PsSPL 3、PsSPL 9、PsSPL 10、PsSPL 13和PsSPL 13 A基因在不同发育芽和种子中的表达谱表明，PsSPL 2、PsSPL 3、PsSPL 9、PsSPL 10、PsSPL 13和PsSPL 13 A是调节牡丹开花时间的重要基因; PsSPL 2和PsSPL 8可能具有抑制侧芽发育的作用，PsSPL 2、PsSPL 13和PsSPL 14对籽粒大小和数量以及角果分支有正控制作用。这些结果为进一步研究PsSPL基因在牡丹生长发育中的功能奠定了基础。
Key messageA total of 16PsSPLgenes were identified in tree peony.PsSPLspotentially regulated flowering time, lateral bud and seed development, and the juvenile-to-adult phase transition.AbstractSQUAMOSA PROMOTER BINDING PROTEIN-LIKE(SPL) transcription factors are important for plant growth and development. Here, we report the identification of 16 full-lengthPsSPLsin tree peony (Peaonia suffruticosaAndr.) and 9PsSPLsthat have miR156 target sites. Phylogenetic analysis of the relationship of SPLs inP. suffruticosaandArabidopsissuggested that they can be classified into six groups, andPsSPLswere highly correlated withArabidopsisSPLs counterparts in the same group.Cis-element of promoter region analysis suggested thatPsSPLgenes play roles in physiological processes and developmental events. Expression analysis indicated that mostPsSPLgenes exhibited high expression levels in the tissues and organs examined here. The increasing expression levels ofPsSPL1,PsSPL2,PsSPL8,PsSPL9,PsSPL12,andPsSPL16, and decreasing expression levels ofPsSPL1AandPsSPL1Bin buds over time suggested that they were probably regulated by the juvenile-to-adult phase transition. In addition, the expression profiles ofPsSPLgenes in different developmental buds and seeds suggested thatPsSPL2,PsSPL3,PsSPL9,PsSPL10,PsSPL13, andPsSPL13Awere important genes for regulating the flowering time of the tree peony;PsSPL2andPsSPL8might play a role in suppressing lateral bud development, andPsSPL2,PsSPL13, andPsSPL14positively controlled grain size and number, and pod branching. These results provide a foundation for future functional analysis ofPsSPLgenes in tree peony growth and development.