Design, Assembly, and Characterization of TALE-Based Transcriptional Activators and Repressors.
Design, Assembly, and Characterization of TALE-Based Transcriptional Activators and Repressors.
复制标题
DOI：
10.1007/978-1-4939-2932-0_7
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Gersbach CA
中科院分区：
文献类型：
--
作者：
Thakore PI;Gersbach CA
Transcription activator-like effectors (TALEs) are modular DNA-binding proteins that can be fused to a variety of effector domains to regulate the epigenome. Nucleotide recognition by TALE monomers follows a simple cipher, making this a powerful and versatile method to activate or repress gene expression. Described here are methods to design, assemble, and test TALE transcription factors (TALE-TFs) for control of endogenous gene expression. In this protocol, TALE arrays are constructed by Golden Gate cloning and tested for activity by transfection and quantitative RT-PCR. These methods for engineering TALE-TFs are useful for studies in reverse genetics and genomics, synthetic biology, and gene therapy.
