Determination of two potential toxicity metabolites derived from the disruption of the pksCT gene in Monascus aurantiacus Li As3.4384
Determination of two potential toxicity metabolites derived from the disruption of the pksCT gene in Monascus aurantiacus Li As3.4384
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测定金红曲霉 Li As3.4384 中 pksCT 基因破坏产生的两种潜在毒性代谢物
DOI：
10.1002/jsfa.8291
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发表时间：
2017
影响因子：
4.1
通讯作者：
Li Yanping
中科院分区：
文献类型：
--
作者：
Huang Zhibing;Su Baowei;Xu Yang;Li Laisheng;Li Yanping
背景我们之前证明了Monascus的pksCT基因的破坏导致Monascus（PHDS 26）的桔霉素生产能力下降98%以上。在pksCT基因被破坏的Monclerus的发酵产物中发现了两种潜在的有毒化合物，monascopyridine A（MPA）和monascopyridine B（MP B）。此外，建立了一种快速可靠的同时测定MPA和MPB的高效液相色谱法。结果表明，最佳提取工艺条件为：甲醇浓度90%，提取温度40 °C，提取时间10 min，提取2次，料液比1：25。在最佳色谱条件下，MPA和MPB分别在0.5-200 μg mL− 1和0.5-300 μg mL− 1浓度范围内呈良好的线性关系，相关系数分别为0.9999和0.9997。MPA的日内和日间精密度的相对标准偏差百分比值分别为2.0%和2.1%; MPB的相应值分别为4.8%和4.6%。MPA和MPB的平均回收率分别为99.9%和94%.CONCLUSION最大MPA和MPB产量（2073.7和1961.7 µg g-1，分别）后观察到16天的培养。© 2017化学工业协会
BACKGROUNDWe previously demonstrated that disruption of thepksCTgene ofMonascusled to a greater than 98% decrease in its citrinin production capacity inMonascus(PHDS26). Two potentially toxic compounds, monascopyridine A (MPA) and monascopyridine B (MPB), were found in the fermentation products of thepksCTgene‐disruptedMonascus. Moreover, a rapid and reliable high‐performance liquid chromatography method was developed for the simultaneous determination of MPA and MPB. We studied the effects of various extraction parameters and designed an orthogonal experiment to investigate the importance of each factor.RESULTSThe optimal extraction conditions were: methanol concentration, 90%; extraction temperature, 40 °C; extraction time, 10 min; two extraction cycles; and a solid‐liquid ratio of 1:25. Under the optimal chromatographic conditions, good linearity was reached over the concentration ranges 0.5–200 µg mL−1and 0.5–300 µg mL−1for MPA and MPB, respectively, and the corresponding determination coefficients were 0.9999 and 0.9997. The percentage relative standard deviation values of within‐day and between‐day precision for MPA were 2.0% and 2.1%, respectively; the corresponding values for MPB were 4.8% and 4.6%. The average recovery for MPA and MPB was 99.9% and 94%, respectively.CONCLUSIONMaximum MPA and MPB yields (2073.7 and 1961.7 µg g−1, respectively) were observed after 16 days of cultivation. © 2017 Society of Chemical Industry