Structural studies of protein-nucleic acid interactions.
Structural studies of protein-nucleic acid interactions.
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蛋白质-核酸相互作用的结构研究。
DOI：
10.1146/annurev.bb.12.060183.001355
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Matthews,BW
中科院分区：
文献类型：
--
作者：
Ohlendorf,DH;Matthews,BW
最近，已经确定了三种调节基因表达的蛋白质的结构：来自λ噬菌体的Cro阻遏蛋白，也来自λ噬菌体的cI或λ阻遏蛋白，以及来自大肠杆菌的分解代谢物基因激活蛋白。这些蛋白质中的每一种都特异性地结合双链DNA上的位点，这些位点通过结合区域内的碱基对序列来区分。这些结构的知识已经抛出了新的光基因调控的许多重要方面，本文综述了在这一领域的最新进展。它讨论了Cro，CAP和CI的结构研究，这些研究提出了一些DNA-蛋白质识别和基因表达的正调控和负调控的一般原则，包括对称性，α-螺旋折叠，序列特异性识别，灵活性，DNA确认，负和正基因调控。
Recently the structures of three proteins that regulate gene expression have been determined: the Cro repressor protein from bacteriophage lambda, the cI or lambda repressor protein, also from phage lambda, and the catabolite gene activator protein from Escherichia coli. Each of these proteins binds specifically to sites on double-stranded DNA that are distinguished by the sequence of base pairs within the binding region. Knowledge of these structures has thrown new light on many of the important aspects of gene regulation, and this paper reviews recent progress in this area. It discusses structural studies of Cro, CAP, and cI which suggest a number of general principles for DNA-protein recognition and for positive and negative regulation of gene expression, including symmetry, alpha-helical fold, sequence-specific recognition, flexibility, DNA confirmation, negative and positive gene regulation.