A locust DNA-binding protein involved in gene regulation by juvenile hormone
A locust DNA-binding protein involved in gene regulation by juvenile hormone
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DOI：
10.1016/s0303-7207(01)00602-5
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发表时间：
2002-04
影响因子：
4.1
通讯作者：
S. Zhou;J. Zhang;M. Hirai;Y. Chinzei;H. Kayser;G. R. Wyatt;V. Walker
中科院分区：
文献类型：
--
作者：
S. Zhou;J. Zhang;M. Hirai;Y. Chinzei;H. Kayser;G. R. Wyatt;V. Walker
尽管保幼激素(JH)在昆虫的发育和繁殖中起着重要的作用，但JH调控基因的分子机制仍然是一个谜。在蝗虫中，位于JH诱导基因jhp21上游的部分回文序列GAGGTTCGAGA/TCCTT/C被认为是一个可能的保幼激素反应元件(JHRE)。当JH缺乏的成虫用活性JH类似物甲基丁二烯处理时，24 h后出现了与JHRE特异结合的脂肪体核因子，结合表现为在左半部与GAGGTTC的反向重复序列、单核苷酸间隔区和可接受某种变异的右半部。与JHRE的结合被核抽提物中存在的C型蛋白激酶催化的磷酸化所取消。因此，DNA结合蛋白被认为是一种转录因子，通过JH的作用使其处于激活状态，然后参与某些JH依赖基因的调节。
Although juvenile hormone (JH) has essential roles in insect development and reproduction, the molecular mechanisms of gene regulation by JH remain an enigma. In Locusta migratoria, the partially palindromic 15-nt sequence, GAGGTTCGAGA/TCCTT/C, found upstream of a JH-induced gene, jhp21, was designated as a putative juvenile hormone response element (JHRE). When JH-deprived adult female locusts were treated with the active JH analog, methoprene, a fat body nuclear factor that bound specifically to JHRE appeared after 24 h. Binding exhibited a preference for an inverted repeat with GAGGTTC in the left half-site, a single nucleotide spacer, and a right half-site in which some variation is acceptable. Binding to JHRE was abolished by phosphorylation catalyzed by a C-type protein kinase present in the nuclear extracts. The DNA-binding protein is thus believed to be a transcription factor, which is brought to an active state through the action of JH and then participates in the regulation of certain JH-dependent genes.