Phytochemical and cytotoxic studies on the roots of Euphorbia fischeriana
Phytochemical and cytotoxic studies on the roots of Euphorbia fischeriana
复制标题
大戟根的植物化学和细胞毒性研究
DOI：
10.1016/j.bmcl.2016.11.063
复制
发表时间：
2017-01-15
影响因子：
2.7
通讯作者：
Meng, Dali
中科院分区：
文献类型：
--
作者：
Shi, Qun;Sun, Yi-wei;Meng, Dali
为支持狼毒的民间应用，对其进行了植物化学研究，共发现9个化合物，其中3个为新化合物(1-3)，6个为已知化合物(4-9)。其结构经一维、二维核磁共振和HRESIMS确证。在对HEP-3B细胞的细胞毒实验中，2对HEP-3B细胞有较强的抑制作用(IC50为8.1mU/L)，1、8和9也有不同程度的抑制作用，IC50值分别为12.5、12.0和18.7mU/L。而在A549细胞上，1(IC5011.9mU/L)和8(IC509.4mU/L)的细胞毒作用优于5-FU，4和9中等，IC50值分别为28.2mU/L和29.8mU/L。此外，石油醚和二氯甲烷提取物均有不同程度的细胞毒作用，而正丁醇提取物则无此作用。结果表明，三萜类化合物、松烷类和三烯类二萜类化合物等低极性组分可能是具有较强抗肿瘤作用的生物活性成分。(C)2016爱思唯尔有限公司。保留所有权利。
With the aim of supporting the folk applications of Euphorbia fischeriana, a phytochemical study was performed, which led to the discovery of 9 compounds, including three new ones (1-3) and six known ones (4-9). Their structures were determined by 1D, 2D NMR, and HRESIMS analysis. In the cytotoxic assays on Hep-3B cell line, 2 showed stronger inhibitory effects (IC50 8.1 mu mol/L) than that of positive control, and 1, 8 and 9 also gave inhibitory effects in a certain degree with IC50 values of 12.5, 12.0 and 18.7 mu mol/L, respectively. While on A549, the cytotoxic activities of 1 (IC50 11.9 mu mol/L) and 8 (IC50 9.4 mu mol/L) were superior to that of 5-Fu, and those of 4 and 9 were moderate with IC50 values of 28.2 and 29.8 mu mol/L, respectively. In addition, both petroleum ether and dichloromethane extracts showed cytotoxic activities with different degree, while n-butanol extracts had no effect. The results clarified that the low-polarity fractions of E. fischeriana, including triterpenoids, abietane and tigliane-type diterpenoids might be the potential bioactive ingredients which will exert strong antitumor effects. (C) 2016 Elsevier Ltd. All rights reserved.