Determination of icariin and metabolites in rat serum by capillary zone electrophoresis: rat pharmacokinetic studies after administration of icariin
Determination of icariin and metabolites in rat serum by capillary zone electrophoresis: rat pharmacokinetic studies after administration of icariin
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DOI：
10.1016/j.jpba.2004.06.021
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发表时间：
2004-10-29
影响因子：
3.4
通讯作者：
Lou, YJ
中科院分区：
文献类型：
--
作者：
Liu, J;Lou, YJ
一种通过毛细管区带电泳测定淫羊藿苷 (ICA) 及其两种代谢物淫羊藿素 (ICT) 和去甲基淫羊藿素 (DICT) 的简单而快速的方法已经开发出来并得到验证。使用四硼酸钠 (30 mmol/L)、磷酸二氢钠 (50 mmol/L)-乙腈 (50:50, v/v) (pH 10.0) 作为运行缓冲液，在 43.6 cm x 50 mm 毛细管上获得 ICA、ICT 和 DICT 的最佳分离效果。卡马西平 (CMP) 用作内标 (IS)。温度和电压分别在 25 摄氏度和 12 W 下进行优化。 270 nm 紫外检测 ICA 的检出限为 1.0 mg/L (S/N = 3)。给健康大鼠单剂量 120 mg ICA/kg 后，对详细方法进行了体内测试。计算的参数证实了该方法在 ICA 大鼠药代动力学研究中的有用性。 (C) 2004 Elsevier B.V. 保留所有权利。
A simple and rapid method for determination of icariin (ICA) and its two metabolites, icaritin (ICT) and desmethylicaritin (DICT), by capillary zone electrophoresis has been developed and validated. Optimum separation of ICA, ICT, and DICT was obtained on 43.6 cm x 50 mum capillary using sodium tetraborate (30 mmol/L), monobasic sodium phosphate (50 mmol/L)-acetonitrile (50:50, v/v) (pH 10.0) as running buffer. Carbamazepine (CMP) was used as internal standard (IS). The temperature and voltage were optimized at 25 degreesC and 12 W, respectively. The limit of detection of ICA was 1.0 mg/L (S/N = 3) by UV detection at 270 nm. The elaborated method was tested in vivo after administration of a single dose of 120 mg ICA/kg to healthy rats. Calculated parameters confirmed usefulness of the method in rat pharmacokinetic studies on ICA. (C) 2004 Elsevier B.V. All rights reserved.