Identification of equine major histocompatibility complex haplotypes using polymorphic microsatellites
Identification of equine major histocompatibility complex haplotypes using polymorphic microsatellites
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DOI：
10.1111/j.1365-2052.2010.02125.x
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发表时间：
2010-12-01
期刊：
影响因子：
2.4
通讯作者：
Antczak, D. F.
中科院分区：
文献类型：
--
作者：
Tseng, C. T.;Miller, D.;Antczak, D. F.
基于位于ECA 20上MHC区域内的5个多态性微卫星，建立了马主要组织相容性复合体（MHC）单倍型鉴定系统。从353匹马的分型中识别出50个微卫星单倍型的分子标记。其中，23个微卫星单倍型与12个已建立的马白细胞抗原（ELA）单倍型在纯种马和标准马。五个ELA血清型与多个微卫星亚单倍型，扩大了马MHC多样性的估计。血清学和微卫星分型之间的强相关性证明了与已知的MHC I类蛋白多态性的联系，并验证了该测定作为ELA血清分型的有用补充，并且在某些应用中，是马家族和人口研究中MHC基因分型的可行替代方法。
P>A system for identifying equine major histocompatibility complex (MHC) haplotypes was developed based on five polymorphic microsatellites located within the MHC region on ECA 20. Molecular signatures for 50 microsatellite haplotypes were recognized from typing 353 horses. Of these, 23 microsatellite haplotypes were associated with 12 established equine leucocyte antigen (ELA) haplotypes in Thoroughbreds and Standardbreds. Five ELA serotypes were associated with multiple microsatellite subhaplotypes, expanding the estimates of diversity in the equine MHC. The strong correlations between serological and microsatellite typing demonstrated a linkage to known MHC class I protein polymorphisms and validated this assay as a useful supplement to ELA serotyping, and in some applications, a feasible alternative method for MHC genotyping in horse families and in population studies.