Molecular Characterization of Vitellogenin and Its Receptor in Sogatella furcifera, and Their Function in Oocyte Maturation
Molecular Characterization of Vitellogenin and Its Receptor in Sogatella furcifera, and Their Function in Oocyte Maturation
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白背飞虱卵黄蛋白原及其受体的分子特征及其在卵母细胞成熟中的功能
DOI：
10.3389/fphys.2019.01532
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发表时间：
2019-12-19
影响因子：
4
通讯作者：
Li, Youzhi
中科院分区：
文献类型：
--
作者：
Hu, Kui;Tian, Ping;Li, Youzhi
卵黄蛋白前体卵黄蛋白原（Vg）为胚胎发育提供营养，而卵黄蛋白原受体（VgR）负责成熟卵母细胞对卵黄蛋白的摄取。这两种蛋白质是昆虫生殖相关的关键蛋白质。本研究克隆并鉴定了白背飞虱Vg和VgR基因，并研究了它们在卵母细胞成熟过程中的功能。克隆的SfVg和SfVgR具有6，114和5，796 bp的开放阅读框，分别编码2，037和1，931个氨基酸残基。结构分析表明，SfVg具有LPD_N、DUF 1943和VWFD三个保守结构域，SfVgR具有LDLR超家族的所有保守基序。这两个基因在成年女性中高度表达; SfVg在脂肪体中表达最高，而SfVgR主要在卵巢中表达。敲除这两个基因都可以减少卵母细胞中卵黄蛋白的沉积，并阻止卵母细胞的成熟。然而，沉默这两个基因中的一个并不影响另一个的转录水平。这些结果证明了SfVgR在将SfVg转运到卵母细胞中的作用。SfVg和SfVgR对卵母细胞的成熟都是必需的。furcifera和这两个基因可以潜在地作为控制这种害虫的手段。
The yolk protein precursor, vitellogenin (Vg), provides nutrition for embryonic development whereas the vitellogenin receptor (VgR) is responsible for the uptake of yolk protein by maturing oocytes. These two proteins are key reproduction-related proteins in insects. We cloned and characterized Vg and VgR genes in Sogatella furcifera, and investigated their function in oocyte maturation. Cloned SfVg and SfVgR have open reading frames of 6,114 and 5,796 bp, encoding 2,037 and 1,931 amino acid residues, respectively. Structural analysis indicates that SfVg has the three conserved LPD_N, DUF1943, and VWFD domains, SfVgR contains all conservative motifs of the LDLR superfamily. Both genes were highly expressed in adult females; SfVg was most highly expressed in the fat body whereas SfVgR was mainly expressed in the ovary. Knockdown of either gene reduced yolk protein deposition in oocytes and arrested oocyte maturation. However, silencing one of these two genes did not affect the transcript level of the other. These results demonstrate the role of SfVgR in transporting SfVg into oocytes. Both SfVg and SfVgR are essential for oocyte maturation in S. furcifera and both genes could potentially be targeted as means of controlling this pest.