Cerberus-like is a secreted factor with neuralizing activity expressed in the anterior primitive endoderm of the mouse gastrula
Cerberus-like is a secreted factor with neuralizing activity expressed in the anterior primitive endoderm of the mouse gastrula
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DOI：
10.1016/s0925-4773(97)00125-1
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发表时间：
1997-11-01
影响因子：
2.6
通讯作者：
De Robertis, EM
中科院分区：
文献类型：
--
作者：
Belo, JA;Bouwmeester, T;De Robertis, EM
我们报告了小鼠 cerberus 样 (cer-l) 的分离，这是一种编码新型分泌蛋白的基因，该蛋白在原肠胚形成早期在前内脏内胚层中特异性表达。原始内胚层中的表达在原条出现之前开始，并持续到头折阶段。在后期阶段，在新形成的体节中发现了第二个表达区域。小鼠 cer-1 与 Xenopus cerberus (Xcer) 具有一些序列相似性。在非洲爪蟾检测 cer-1 中，与 Xcer 一样，mRNA 充当有效的神经化因子，诱导前脑标记和内胚层，但无法像 Xcer 那样诱导异位头状结构。除了 cer-E 之外，前内脏内胚层还表达转录因子 Lim1、goosecoid 和 HNF-3 beta，这些因子也存在于躯干组织细胞中。讨论了如何调节头部和躯干发育的模型。鉴于其神经化活性，分泌蛋白 Cer-1 是介导前内脏内胚层诱导活性的候选蛋白。 (C) 1997 爱思唯尔科学爱尔兰有限公司
We report the isolation of mouse cerberus-like (cer-l), a gene encoding a novel secreted protein that is specifically expressed in the anterior visceral endoderm during early gastrulation. Expression in the primitive endoderm starts before the appearance of the primitive streak and lasts until the head-fold stage. In later stages, a second region of expression is found in newly formed somites. Mouse cer-l shares some sequence similarity with Xenopus cerberus (Xcer). In Xenopus assays cer-l, like Xcer, mRNA acts as a potent neuralizing factor that induces forebrain markers and endoderm, but is unable to induce ectopic head-like structures as Xcer does. In addition to cer-E, anterior visceral endoderm was found to express the transcription factors Lim1, goosecoid and HNF-3 beta that are also present in trunk organizer cells. A model of how head and trunk development might be regulated is discussed. Given its neuralizing activity, the secreted protein Cer-l is a candidate for mediating inductive activities of anterior Visceral endoderm. (C) 1997 Elsevier Science Ireland Ltd.