Specificity of Distalless repression and limb primordia development by abdominal Hox proteins
Specificity of Distalless repression and limb primordia development by abdominal Hox proteins
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DOI：
10.1016/s1534-5807(02)00257-5
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发表时间：
2002-10-01
影响因子：
11.8
通讯作者：
Mann, RS
中科院分区：
文献类型：
--
作者：
Gebelein, B;Culi, J;Mann, RS
在果蝇中，节段之间的差异，如附件的存在或不存在，由Hox转录因子控制。Hox蛋白通过抑制腿选择基因Distalless而抑制腹部肢体的形成，而胸部Hox蛋白Antennapedia(ANTP)则不抑制Distalless。我们发现，HOX辅助因子；牙外环和同胸选择性地增强UBX，而不是ANTP，与一个无距离调节序列结合。Ubx中的一个C-末端多肽刺激与这个位点的结合。然而，DNA结合不足以进行无距离抑制。相反，在Ubx中需要一个额外的选择性剪接结构域来进行无距离抑制，但不需要DNA结合。因此，HOX蛋白的功能特异性取决于DNA结合依赖和非依赖机制。
In Drosophila, differences between segments, such as the presence or absence of appendages, are controlled by Hox transcription factors. The Hox protein Ultrabithorax (Ubx) suppresses limb formation in the abdomen by repressing the leg selector gene Distalless, whereas Antennapedia (Antp), a thoracic Hox protein, does not repress Distalless. We show that the Hox cofactors; Extradenticle and Homothorax selectively enhance Ubx, but not Antp, binding to a Distalless regulatory sequence. A C-terminal peptide in Ubx stimulates binding to this site. However, DNA binding is not sufficient for Distalless repression. Instead, an additional alternatively spliced domain in Ubx is required for Distalless repression but not DNA binding. Thus, the functional specificities of Hox proteins depend on both DNA binding-dependent and -independent mechanisms.