NOVEL POTENTIAL MITOTIC MOTOR PROTEIN ENCODED BY THE FISSION YEAST CUT7+ GENE
NOVEL POTENTIAL MITOTIC MOTOR PROTEIN ENCODED BY THE FISSION YEAST CUT7+ GENE
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DOI：
10.1038/347563a0
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发表时间：
1990-10-11
期刊：
影响因子：
64.8
通讯作者：
YANAGIDA, M
中科院分区：
文献类型：
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作者：
HAGAN, I;YANAGIDA, M
在分裂酵母中，与高等真核中心体相当的结构，核膜结合的纺锤体极体，在分裂间期是不活跃的。在细胞周期从G2期过渡到M期时，纺锤体极体复制;子极体播种微管，其相互交叉形成短纺锤体，其在中期1 -5伸长以跨越细胞核。我们已经确定了两个基因座，当突变时，阻止纺锤体形成。这些基因之一的预测产物cut 7+（参考文献6）含有一个类似于驱动蛋白重链头部结构域的氨基末端结构域7，8，表明cut 7+产物可能是一个纺锤体马达。cut 7+基因类似于构巢曲霉纺锤体运动基因bimC（参考文献9），无论是在其组织与同源的氨基末端头部和没有明显的七肽重复序列，并在突变表型的形态。但我们发现这些基因的羧基末端之间没有相似性，这表明要么thecut 7+基因代表一类新的驱动蛋白基因，并且裂殖酵母可能另外含有abimChomologue，要么这些有丝分裂驱动蛋白的羧基末端不是进化保守的，并且thecut 7+基因属于bimC相关驱动蛋白的一个亚组。
THE structure equivalent to higher eukaryotic centrosomes in fission yeast, the nuclear membrane-bound spindle pole body, is inactive during interphase. On transition from G2 to M phase of the cell cycle, the spindle pole body duplicates; the daughter pole bodies seed microtubules which interdigitate to form a short spindle that elongates to span the nucleus at metaphase1–5. We have identified two loci which, when mutated, block spindle formation. The predicted product of one of these genes,cut7+(ref. 6), contains an amino-terminal domain similar to the kinesin heavy chain head domain7,8, indicating that thecut7+product could be a spindle motor. Thecut7+gene resembles theAspergillus nidulansputative spindle motor genebimC(ref. 9), both in terms of its organization with a homologous amino-terminal head and no obvious heptad repeats and in the morphology of the mutant phenotype. But we find no similarity between the carboxy termini of these genes, suggesting that either thecut7+gene represents a new class of kinesin genes and that fission yeast may in addition contain abimChomologue, or that the carboxy termini of these mitotic kinesins are not evolutionary conserved and that thecut7+gene belongs to a subgroup ofbimC-related kinesins.