Cellular heterogeneity in normal and neoplastic human urothelium.
Cellular heterogeneity in normal and neoplastic human urothelium.
复制标题
正常和肿瘤性人类尿路上皮的细胞异质性。
作者：
W. Mackillop;J. P. Bizarri;G. Ward
采用不连续密度梯度离心法对2例正常人尿路上皮和13例移行细胞癌的细胞悬液进行了研究。对各组分的组织化学和增殖特性进行了评价。在正常尿路上皮中，DNA合成仅限于小圆细胞的亚群，这些细胞可以根据其较高的物理密度与更多的锥体细胞和巨细胞部分分离。正常尿路上皮细胞在琼脂中不形成菌落。在分化良好的肿瘤中，大多数细胞是低密度的，细长的细胞，其组织化学上类似于正常组织的分化的锥体细胞，但DNA合成和在琼脂中形成集落的能力仅限于高密度小圆细胞的亚群。集落大小分析表明，中等密度的条带含有能够进行少量分裂的细胞，这些细胞不会形成大的集落。随着肿瘤级别的增加，有一个明显的转移到高密度克隆形成室。
Cell suspensions derived from 2 specimens of normal human urothelium and 13 human transitional cell carcinomas were studied by discontinuous density gradient centrifugation. The histochemical and proliferative properties of the fractions were evaluated. In normal urothelium, DNA synthesis was restricted to a subpopulation of small round cells which could be partly separated from the more numerous pyramidal and giant cells on the basis of their higher physical density. Normal urothelium did not form colonies in agar. In well-differentiated tumors, the majority of cells present were low-density, elongated cells which histochemically resembled the differentiated pyramidal cells of normal tissue, but DNA synthesis and the ability to form colonies in agar were restricted to a subpopulation of high-density small round cells. Colony size analysis showed that intermediate-density bands contain cells capable of a few divisions which do not form large colonies. With increasing tumor grade, there was an apparent shift into the high-density clonogenic compartment.
半固体培养基中肿瘤集落内细胞数量及其扁球体生长的定量。
影响因子：
11.2
作者：
MeyskensJr,FL;Thomson,SP;Moon,TE
通讯作者：
Moon,TE