Coumarin alleviates ovalbumin-induced food anaphylaxis in a mouse model by affecting mast cell function
Coumarin alleviates ovalbumin-induced food anaphylaxis in a mouse model by affecting mast cell function
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香豆素通过影响肥大细胞功能减轻小鼠模型中卵清蛋白诱导的食物过敏反应
DOI：
10.1039/c9fo01776c
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发表时间：
2019
期刊：
影响因子：
6.1
通讯作者：
Guang-Ming Liu
中科院分区：
文献类型：
--
作者：
Qing-Mei Liu;Ya-Fen Zhang;Yuan-Yuan Gao;Hong Liu;Min-Jie Cao;Xian-Wen Yang;Wen-Jin Su;Guang-Ming Liu
香豆素是一种重要的有机杂环化合物，在自然界中来源广泛。由于其存在于多种生物活性化合物中，具有广泛的生物活性，在药物发现过程中发挥着重要作用。本研究采用卵清蛋白（OVA）诱导的小鼠食物过敏模型和免疫球蛋白（IG）E介导的小鼠骨髓源性肥大细胞（BMMC）模型评价香豆素的抗过敏活性。香豆素可减轻OVA致敏小鼠的过敏症状，降低腹泻率，促进直肠温度升高。此外，香豆素有能力降低组胺和小鼠肥大细胞蛋白酶的水平，抑制OVA特异性IgE，并显着减少脾脏和肠系膜淋巴结中肥大细胞的数量。香豆素还能显著抑制肥大细胞依赖的被动皮肤过敏反应。此外，由于香豆素引起c-KIT受体的抑制，成熟BMMC的数量减少。此外，香豆素以浓度依赖性方式上调OVA激活的BMMCs的凋亡。结论：香豆素通过调节肥大细胞的功能和数量，显示出有效的抗食物过敏活性。香豆素及其衍生物为抗食物过敏药物成分的开发提供了新的方向。
Coumarin is an important organic heterocyclic compound with a wide range of sources in nature. It plays an important role in the drug discovery process due to its existence in diverse biologically active compounds and its broad bioactivity. In this study, the anti-allergic activity of coumarin was evaluated using an ovalbumin (OVA)-induced mouse food allergy model and an immunoglobulin (Ig)E mediated mouse bone marrow-derived mast cell (BMMC) model. Coumarin could alleviate the OVA-induced allergic symptoms, decrease the diarrhea rates, and promote the rectal temperature rise in allergic mice. Moreover, coumarin had the ability to reduce the levels of histamine and mouse mast cell proteinases, inhibit OVA-specific IgE, and significantly decrease the population of mast cells in the spleen and mesenteric lymph nodes. Coumarin could also significantly suppress mast cell-dependent passive cutaneous anaphylaxis. Additionally, the number of mature BMMCs was decreased as coumarin caused the suppression of c-KIT receptors. Furthermore, coumarin up-regulated the apoptosis of OVA-activated BMMCs in a concentration-dependent manner. In conclusion, coumarin displayed effective anti-food allergy activity via the regulation of mast cell function and numbers. Coumarin and its derivatives provide a new direction for the development of anti-food allergic drug components.