Protein Synthesis Assisted by Native Chemical Ligation at Leucine
Protein Synthesis Assisted by Native Chemical Ligation at Leucine
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DOI：
10.1002/cbic.201000168
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发表时间：
2010-06-14
期刊：
影响因子：
3.2
通讯作者：
Brik, Ashraf
中科院分区：
文献类型：
--
作者：
Harpaz, Ziv;Siman, Peter;Brik, Ashraf
（化学方程式）触发亮氨酸：开发了一种使用β-巯基亮氨酸与亮氨酸位点的脱硫偶联的新连接策略，并提出了其在蛋白质合成中的适用性。我们的Leu-NCL的效率在几种模型肽中进行了检测，并用于HIV-1达特蛋白的首次全合成。
(Chemical Equation Presented) Triggering leucine: A new ligation strategy of using β-mercaptoleucine coupled with desulfurization at leucine sites was developed, and its applicability in protein synthesis is presented. The efficiency of our Leu-NCL was examined in several model peptides and utilized for the first total synthesis of HIV-1 Tat protein.