Histone deacetylase complex1 expression level titrates plant growth and abscisic acid sensitivity in Arabidopsis.
Histone deacetylase complex1 expression level titrates plant growth and abscisic acid sensitivity in Arabidopsis.
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组蛋白脱乙酰酶复合物 1 表达水平滴定拟南芥植物生长和脱落酸敏感性。
DOI：
10.1105/tpc.113.114835
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Perrella G
中科院分区：
文献类型：
--
作者：
Perrella G
组蛋白去乙酰化在植物胁迫响应过程中调节基因表达，因此是植物胁迫敏感性表观遗传操纵的一个有趣的靶点。不幸的是，核心酶（组蛋白脱乙酰基酶[HDAC]）的过度表达要么无效，要么导致多效性形态异常。在酵母和哺乳动物中，HDAC在多蛋白复合物中起作用。寻找植物HDAC复合物的推定成分，我们确定了一个基因与酵母复合物的功能不明的成员，我们称之为组蛋白脱乙酰化复合物1（HDC1）的部分同源性。HDC1由模式植物和作物基因组中的单拷贝基因编码，因此为生物技术提供了有吸引力的靶标。在这里，我们提出了一个功能特性的HDC1在拟南芥。我们发现，HDC1是一种普遍表达的核蛋白，与至少两种脱乙酰酶（HDA6和HDA19）相互作用，促进组蛋白脱乙酰化，并减弱水分胁迫下基因的去阻遏。快速生长的HDC1过表达植物不仅在水分充足的土壤上表现优于野生型植物，而且在供水减少时也表现优于野生型植物。我们的研究结果确定HDC1作为组蛋白脱乙酰化机制的限速成分，并作为一个有吸引力的工具，提高植物的发芽率和生物量生产。
Histone deacetylation regulates gene expression during plant stress responses and is therefore an interesting target for epigenetic manipulation of stress sensitivity in plants. Unfortunately, overexpression of the core enzymes (histone deacetylases [HDACs]) has either been ineffective or has caused pleiotropic morphological abnormalities. In yeast and mammals, HDACs operate within multiprotein complexes. Searching for putative components of plant HDAC complexes, we identified a gene with partial homology to a functionally uncharacterized member of the yeast complex, which we called Histone Deacetylation Complex1 (HDC1). HDC1 is encoded by a single-copy gene in the genomes of model plants and crops and therefore presents an attractive target for biotechnology. Here, we present a functional characterization of HDC1 inArabidopsis thaliana. We show that HDC1 is a ubiquitously expressed nuclear protein that interacts with at least two deacetylases (HDA6 and HDA19), promotes histone deacetylation, and attenuates derepression of genes under water stress. The fast-growing HDC1-overexpressing plants outperformed wild-type plants not only on well-watered soil but also when water supply was reduced. Our findings identify HDC1 as a rate-limiting component of the histone deacetylation machinery and as an attractive tool for increasing germination rate and biomass production of plants.