Immune response to Newcastle disease virus in chicken lines divergently selected for cutaneous hypersensitivity
Immune response to Newcastle disease virus in chicken lines divergently selected for cutaneous hypersensitivity
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DOI：
10.1111/j.1744-313x.2007.00722.x
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发表时间：
2007-12-01
影响因子：
2.2
通讯作者：
Rasool, T. J.
中科院分区：
文献类型：
--
作者：
Ahmed, K. A.;Saxena, V. K.;Rasool, T. J.
本文首次报道了细胞免疫不同的禽类对新城疫强毒(NDV)的不同免疫反应，通过对植物血凝素P的反应来衡量。为了探索潜在的宿主-病原体相互作用，采集了40只极端应答鸟的外周血单核细胞(PBMC)(分别来自高和低细胞免疫品系的20只鸟)。用新城疫强毒和干扰素-γ刺激PBMC培养，用半定量逆转录-聚合酶链式反应检测诱导型一氧化氮合酶(INOS)的表达。为了进一步探讨诱导型一氧化氮合酶mRNA表达与一氧化氮(NO)产生的关系，我们检测了培养上清液中NO的含量。细胞免疫水平越高，NO产量越高，iNOS和干扰素-γ的表达也越高(P<；0.05)。在我们的研究中，在TspEI位点的干扰素-γ启动子多态性的品系之间观察到了显著的差异(P<；0.05)。高细胞免疫株主要表现为168bp的GG基因片段。另一方面，该基因在细胞免疫水平较低的品系中并不占优势。随后，实时定量聚合酶链式反应显示GG基因对新城疫病毒有较高的转录水平(P<；0.01)。这种启动子区域的差异可能是鸡不同品系干扰素-γ基因转录差异的原因。此外，高细胞免疫品系的鸡在加强新城疫疫苗免疫方面表现出更好的适应性免疫，抗体效价也有所提高。因此，这项研究提供了基于植物血凝素-P反应的选择对新城疫强毒免疫反应影响的基线数据，这些数据对家禽遗传学家和免疫学家试图培育家禽抗病能力具有极其重要的意义。
This paper describes for the first time the differential immune response to virulent Newcastle disease virus (NDV) in birds differing in cell-mediated immunity, as measured by response to phytohaemagglutinin-P. To explore potential host-pathogen interactions, peripheral blood mononuclear cells (PBMC) were collected from 40 extreme responder birds (20 birds each from high and low cell-mediated immunity lines). PBMC cultures were stimulated by virulent NDV and temporal expression profiles of interferon-gamma (IFN-gamma), and inducible nitric oxide synthase (iNOS) mRNA was evaluated by semiquantitative reverse transcription polymerase chain reaction (PCR). To further explore the correlation of iNOS mRNA expression and nitric oxide (NO) production, we assayed the culture supernatants for NO. NO production, as well as iNOS and IFN-gamma mRNA expression, was significantly (P < 0.05) higher in the line with higher cell-mediated immunity. In our study, a significant (P < 0.05) difference was observed between the lines for IFN-gamma promoter polymorphism for the TspEI site. The high cell-mediated immunity line mostly revealed the genotype (GG) with a 168-bp fragment. On the other hand, this genotype was not predominant in the low cell-mediated immunity line. Later, quantitative real-time PCR demonstrated higher (P < 0.01) IFN-gamma mRNA transcription in the genotype GG in response to NDV. This difference in promoter region may be responsible for differential IFN-gamma mRNA transcription in chicken lines. Furthermore, birds of high cell-mediated immunity line showed better adaptive immunity to booster NDV vaccination as revealed by an enhanced antibody titre. Thus, this study provides baseline data on the effect of phytohaemagglutinin-P response-based selection on immune responses to virulent NDV and the data could be of immense importance to poultry geneticist and immunologist attempting to breed poultry for disease resistance.