The human v-abl cellular homologue.
The human v-abl cellular homologue.
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人类 v-abl 细胞同源物。
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
J. Stephenson
中科院分区：
文献类型：
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作者：
N. Heisterkamp;J. Groffen;J. Stephenson
从用MboI部分消化的人肺癌DNA的代表性文库中分离出了细胞DNA序列的连续区域，长度为64 kb，代表来自三个独立粘粒克隆的重叠细胞插入片段。在细胞基因组的这个区域内，v-abl同源序列分散在约32 kb的总区域上。这些序列代表整个v-abl人细胞同源物，与病毒v-abl转化基因共线，并含有最少7个间插序列。至少有八个区域的高度重复的DNA序列已被证明映射在接近c-abl编码序列。除了主要的c-abl人类基因座之外，已经鉴定了仅对应于v-abl基因的部分的人类DNA序列的三个区域。其中两个已被分子克隆，并显示出与主要的人类c-abl基因座不同。转染培养的大鼠胚胎成纤维细胞后，没有一个含有v-abl同源序列的粘粒DNA表现出转化活性。这些发现确定并绘制了人类DNA的单个遗传位点c-abl，代表完整的v-abl同源物，并证明存在对应于更有限的v-abl亚基因组区域的额外的人类DNA序列。
A contiguous region of cellular DNA sequence, 64 kb in length and representing overlapping cellular inserts from three independent cosmid clones, has been isolated from a representative library of human lung carcinoma DNA partially digested with MboI. Within this region of the cellular genome, v-abl homologous sequences are dispersed over a total region of around 32 kb. These sequences represent the entire v-abl human cellular homologue, are colinear with the viral v-abl transforming gene, and contain a minimum of seven intervening sequences. At least eight regions of highly repetitive DNA sequences have been shown to map in close proximity to c-abl coding sequences. In addition to the major c-abl human locus, three regions of human DNA sequence, corresponding to only portions of the v-abl gene, have been identified. Two of these have been molecularly cloned and shown to be distinct from the primary human c-abl locus. Upon transfection to rat embryo fibroblasts in culture, none of the cosmid DNAs containing v-abl homologous sequences exhibited transforming activity. These findings identify and map a single genetic locus of human DNA, c-abl, representing the complete v-abl homologue, and demonstrate the existence of additional human DNA sequences corresponding to more limited, subgenomic regions of v-abl.