Synthesis of DNA-Binding Peptoids
Synthesis of DNA-Binding Peptoids
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DOI：
10.1055/s-0034-1380698
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发表时间：
2015-07-01
期刊：
影响因子：
2
通讯作者：
Bong, Dennis
中科院分区：
文献类型：
--
作者：
Mao, Jie;Bong, Dennis
通过核酸碱基配对的可编程分子识别已经使得能够使用DNA、RNA、PNA和最近的双面PNA（bPNA）在纳米和生物技术中应用。我们在本文中描述了带有双面合成核碱基三聚氰胺的类肽骨架的合成和DNA识别特性。这些类肽核酸与富含胸腺嘧啶的DNA杂交，如它们的肽同源物（bPNA）。DNA络合对类肽侧链长度和总电荷高度敏感。与肽bPNA同分异构的类肽在DNA识别方面效率较低，可能是由于构象和空间差异。1三嗪类化合物与DNA分子识别2 DNA结合类肽的合成3类肽与DNA的结合研究
Programmable molecular recognition through nucleic acid base pairing has enabled applications in nano- and biotechnology using DNA, RNA, PNA, and more recently, bifacial PNA (bPNA). We describe herein the synthesis and DNA recognition properties of peptoid backbones bearing the bifacial synthetic nucleobase melamine. These peptoid nucleic acids' hybridize with thymine-rich DNA, like their peptide cognate (bPNA). DNA complexation is highly sensitive to peptoid side-chain length and overall charge. Peptoids isomeric with peptide bPNA were less efficient at DNA recognition, possibly due to conformational and steric differences. 1 Triazines and DNA Molecular Recognition 2 Synthesis of DNA-Binding Peptoids 3 Peptoid-DNA Binding Studies