Coronin3 regulates gastric cancer invasion and metastasis by interacting with Arp2
Coronin3 regulates gastric cancer invasion and metastasis by interacting with Arp2
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Coronin3 通过与 Arp2 相互作用调节胃癌侵袭和转移。
DOI：
10.4161/cbt.29501
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发表时间：
2014-01-01
影响因子：
3.6
通讯作者：
Wang, Xin
中科院分区：
文献类型：
--
作者：
Sun, Yi;Shang, Yulong;Wang, Xin
Coronin3在胃癌组织中表达增加，可促进胃癌的侵袭和转移。然而，Coronin3在GC中的作用机制仍不清楚。在这项研究中，我们的目的是探索相互作用的分子至关重要的肿瘤促进作用的冠状蛋白3在GC。通过质谱分析、功能研究和免疫组化，我们发现Arp2与Coronin3相互作用，Arp2的异位表达促进了GC细胞的迁移和侵袭，而Arp2敲低抑制了全细胞的运动，减弱了Coronin3介导的细胞迁移和侵袭的上调。此外，这两种蛋白与胃癌患者的转移状态相关。此外，生存分析表明，Coronin3和Arp2都与GC患者的总体生存相关，Coronin3和Arp2的组合最准确地预测GC患者的预后。这些数据表明，Coronin3可以通过Arp2调节GC的侵袭和转移，Coronin3和Arp2的组合提供了预测GC预后的潜在标志物。
Coronin3 expression is increased in gastric cancer (GC) tissues and can promote GC invasion and metastasis. However, the mechanisms underlying Coronin3 function in GC remain unclear. In this study, we aimed to explore the interacting molecules essential for the tumor-promoting effects of Coronin3 in GC. Using mass spectrometric analysis, functional studies, and immunohistochemistry, we found that Arp2 interacted with Coronin3, and ectopic expression of Arp2 promoted GC cell migration and invasion, while Arp2 knockdown suppressed whole-cell motility and attenuated the Coronin3-mediated upregulation of cell migration and invasion. In addition, both proteins correlated with the metastatic status of GC patients. Furthermore, survival analyses demonstrated that both Coronin3 and Arp2 correlated with overall GC patient survival, and the combination of Coronin3 and Arp2 most accurately predicted GC patient prognosis. Combined, these data demonstrate that Coronin3 can regulate GC invasion and metastasis through Arp2, and the combination of Coronin3 and Arp2 provides a potential marker for predicting GC prognosis.