Leukotriene B4 production by the human alveolar macrophage: a potential mechanism for amplifying inflammation in the lung.
Leukotriene B4 production by the human alveolar macrophage: a potential mechanism for amplifying inflammation in the lung.
复制标题
人肺泡巨噬细胞产生白三烯 B4：放大肺部炎症的潜在机制。
DOI：
10.1164/arrd.1984.129.1.106
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Henderson,WR
中科院分区：
文献类型：
--
作者：
Martin,TR;Altman,LC;Albert,RK;Henderson,WR
白三烯 B4(LTB4) 是花生四烯酸的脂氧合酶产物，对血液白细胞具有有效的趋化活性。为了评估 LTB4 在肺部炎症反应中的潜在作用，我们研究了人肺泡巨噬细胞产生 LTB4，并在体外和体内测定了其对肺和血液吞噬细胞的趋化活性。用钙离子载体 A23187 (10 µg/ml) 刺激人肺泡巨噬细胞，并通过高效液相色谱分离上清液中的脂氧合酶产物。白三烯 B4 是 2 名非吸烟者 (17.3 ± 2.7 ng/106 细胞) 和 4 名吸烟者 (23.4 ± 14.8 ng/106 细胞) 中的 3 名肺泡巨噬细胞的主要花生四烯酸脂氧合酶产物。肺泡巨噬细胞比经过类似处理的外周血中性粒细胞产生更多的 LTB4。受刺激的肺泡巨噬细胞还产生 5-羟基二十四烯酸 (HETE) 和 LTB4 的 2 种异构体：5-(S),12-(R)-6-反式-LTB4 和 5-(S),12-(S)-6-反式-LTB4。白三烯 B4 在体外对肺泡巨噬细胞显示出很小的趋化活性，对外周血中性粒细胞的趋化作用比对单核细胞的作用更强 (p < 0.05)。当滴注到麻醉大鼠的气道中时，LTB4 对中性粒细胞和单核细胞的吸引力不如酵母聚糖激活的血清或细菌衍生的趋化因子。肺泡巨噬细胞产生的白三烯 B4 可能提供了一种机制，通过该机制，吞噬细胞在炎症过程中被募集至人肺。
Leukotriene B4(LTB4) is a lipoxygenase product off arachidonic acid that has potent chemotactlc activity for blood leukocytes. To assess the potential role off LTB4in lung inflammatory responses, we investigated the production off LTB4by human alveolar macrophages and determined its chemotactlc activity for lung and blood phagocytesIn vitroandIn vivo.Human alveolar macrophages were stimulated with the calcium lonophore A23187 (10 µg/ml), and lipoxygenase products in the supernatants were isolated by high-performance liquid chromatography. Leukotriene B4was the predominant arachldonate lipoxygenase product from the alveolar macrophages off 2 nonsmokers (17.3 ± 2.7 ng/106cells) and 3 off 4 smokers (23.4 ± 14.8 ng/106cells). Alveolar macrophages produced more LTB4than did similarly treated peripheral blood neutrophils. Stimulated alveolar macrophages also produced 5-hydroxyelcosatetraenolc acid (HETE) and 2 isomers off LTB4:5-(S),12-(R)-6-trans-LTB4and 5-(S),12-(S)-6-trans-LTB4. Leukotriene B4showed little chemotactlc activity for alveolar macrophagesIn vitroand was a more potent chemoattractant for peripheral blood neutrophils than for monocytes (p < 0.05). When instilled into the airways off anesthetized rats, LTB4was less potent as an attractant for neutrophils and mononuclear cells than either zymosan-activated serum or bacterial-derived chemotactic factors. Leukotriene B4production by alveolar macrophages may provide a mechanism by which phagocytes are recruited to the human lung during inflammatory processes.