Cell wall investigations utilizing tip-enhanced Raman scattering
Cell wall investigations utilizing tip-enhanced Raman scattering
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DOI：
10.1111/j.1365-2818.2008.01939.x
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发表时间：
2008-03-01
影响因子：
2
通讯作者：
Deckert, V.
中科院分区：
文献类型：
--
作者：
Budich, C.;Neugebauer, U.;Deckert, V.
利用尖端增强拉曼散射技术研究了具有高横向分辨率和化学特异性的表皮葡萄球菌细胞壁的表面结构。对于大多数生物样品，透射式尖端增强拉曼散射装置非常适合，因为它允许将样品保存在特定环境条件下，而它可以最有效地收集场增强探头产生的信号。这里特别强调了重复性地设置仪器所需的参数，以便能够正确地估计场增强因子。此外，还将通过一个例子来强调对照实验对于避免对信号的误解的重要性。
Tip-enhanced Raman scattering is used to investigate the surface structure of the cell wall of Staphylococcus epidermidis with high lateral resolution and chemical specificity. For most biological samples, the transmission tip-enhanced Raman scattering set-up is ideally suited because it allows the specimen to be kept under specific environmental conditions, whereas it most efficiently collects the signal created at the field-enhancing probe. Special emphasis is given here to the parameters required to reproducibly set up the instrument, such that field-enhancement factors can be estimated properly. Also the importance of control experiments to avoid misinterpretation of signals will be emphasized by an example.