Heterogeneous distribution of thrombomodulin and von Willebrand factor in endothelial cells in the human pulmonary microvessels
Heterogeneous distribution of thrombomodulin and von Willebrand factor in endothelial cells in the human pulmonary microvessels
复制标题
DOI：
10.1272/jnms.67.118
复制
发表时间：
2000-04-01
影响因子：
1
通讯作者：
Ferrans, Victor J.
中科院分区：
文献类型：
--
作者：
Kawanami, Oichi;Jin, Enjing;Ferrans, Victor J.
采用激光扫描共聚焦荧光显微镜技术研究了正常人肺微血管内皮细胞中血管性血血病因子（vWf； factor viii相关抗原）和凝血调节蛋白（凝血酶跨膜受体）的定位。11例患者单发结节（7例腺癌和4例错构瘤）的肺叶切除标本获得组织。肺泡区（a区）毛细血管内皮细胞的质膜显示血栓调节素的红色线性荧光。然而，它们的细胞质大多对vWf无反应。位于结缔组织（c区）的微血管，包括支气管周围、胸膜下和大血管壁，其细胞质中vWf一致显示带状绿色荧光，其质膜通常对血栓调节素缺乏反应性。只有少数直径< 10mum的支气管周围毛细血管呈镶嵌状，质膜上可见红色荧光，细胞质下可见绿色荧光。在位于A区和c区交界处的j区微血管中，直径可达40 μ m，内皮细胞显示两种抗原呈镶嵌状分布。然而，血栓调节素在j区微血管中的定位与vWf是独立的。血栓调节蛋白反应细胞直接与肺泡毛细血管内皮细胞相连。血栓调节蛋白和vWf分布的不均匀模式表明，在正常人肺中，内皮功能的地形差异是维持凝血和抗凝血平衡的必要条件。
Laser scanning confocal fluorescence microscopy techniques were used to study the localization of von Willebrand factor (vWf; Factor VIII-related antigen) and thrombomodulin (transmembrane receptor for thrombin) in the microvascular endothelial cells in the normal human lung. Tissues were obtained from lobectomy specimens resected for solitary nodules (7 adenocarcinomas and 4 hamartomas) from 11 patients. The plasma membranes of the capillary endothelial cells in the alveolar zones (A-zones) showed red linear fluorescence for thrombomodulin. However, their cytoplasm was mostly unreactive for vWf. The microvessels which were located in the connective tissue (C-zones), including peribronchial, and subpleural areas and large vascular walls, consistently demonstrated band-like green fluorescence for vWf in their cytoplasm, and their plasma membranes usually lacked reactivity for thrombomodulin. Only a limited number of peribronchial capillaries measuring < 10mum in diameter showed a mosaic-like appearance, in which red fluorescence along the plasma membranes was found together with green fluorescence in the subjacent cytoplasm. In the juxtaalveolar (J-zones) microvessels located along the borders between A- and C-zones, and measuring up to 40 mum in diameter, the endothelial cells showed a mosaic-like pattern of distribution of the two antigens. However, the localization of thrombomodulin in the J-zone microvessels was separate and independent from that of vWf. The thrombomodulin-reactive cells were directly connected to the alveolar capillary endothelial cells. Heterogeneous patterns of distribution of thrombomodulin and vWf suggest that topographic differences of endothelial function occur to maintain a balance of coagulation and anticoagulation in the normal human lung.