Quantitative techniques for imaging cells and tissues.
Quantitative techniques for imaging cells and tissues.
复制标题
细胞和组织成像的定量技术。
DOI：
10.1007/s00441-015-2149-0
复制
发表时间：
2015
影响因子：
3.6
通讯作者：
Wouters,FredS
中科院分区：
文献类型：
--
作者：
vonBartheld,ChristopherS;Wouters,FredS
这个特别的问题集中在细胞生物学的两个主要方面：成像和定量。细胞生物学的历史与成像密切相关，特别是在过去几个世纪中与显微镜的进步密切相关（Wolpert 1995; Evanko et al. 2009; Mildier Light Microscopy 2009）。几个世纪前，显微镜的发明有助于定义细胞理论，几十年前，电子显微镜使可视化的边界进一步发展。荧光共聚焦显微镜的应用使得在细胞、亚细胞甚至分子水平上观察的新方法成为可能（目前仍在发展;沃茨and Wittmann 2014）。定量方法在细胞生物学中的重要性较少被认识。量化为科学过程/奋进增加了一个重要的，通常是必不可少的新维度，正如开尔文勋爵的名言所表达的那样：在物理科学中，学习任何学科的第一个重要步骤是找到数值计算的原则和实际测量与之相关的某些质量的方法......当你能测量你所说的东西，并用数字表达它时，你对它有所了解;但是当你不能测量它时，你就知道了。
This special issue focuses on two major aspects of cell biology: imaging and quantitation. The history of cell biology has been tightly interwoven with imaging and in particular with advances in microscopy throughout the past centuries (Wolpert 1995; Evanko et al. 2009; Milestones Light Microscopy 2009). The invention of microscopes was instrumental in defining cell theory, centuries ago, and the boundaries of visualization advanced further with electron microscopy, decades ago. Applications of fluorescent confocal microscopy have enabled new ways of observation at the cellular, subcellular and even molecular level (still evolving to the current time; Waters and Wittmann 2014). Less appreciated is the importance of quantitative approaches in cell biology. Quantitation adds an important, often essential, new dimension to the scientific process/endeavor, as expressed in the famous quote by Lord Kelvin:BIn physical science a first essential step in the direction of learning any subject is to find principles of numerical reckoning and methods for practicably measuring some quality connected with it… when you can measure what you are speaking about, and express it in numbers, you know something about it; but when you cannot