TONSOKU is expressed in S phase of the cell cycle and its defect delays cell cycle progression in Arabidopsis.
TONSOKU is expressed in S phase of the cell cycle and its defect delays cell cycle progression in Arabidopsis.
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DOI：
10.1093/pcp/pci082
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发表时间：
2005-05
影响因子：
4.9
通讯作者：
Takamasa Suzuki;Sakiko Nakajima;Soichi Inagaki;Makiko Hirano-Nakakita;K. Matsuoka;T. Demura;H. Fukuda;A. Morikami;K. Nakamura
中科院分区：
文献类型：
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作者：
Takamasa Suzuki;Sakiko Nakajima;Soichi Inagaki;Makiko Hirano-Nakakita;K. Matsuoka;T. Demura;H. Fukuda;A. Morikami;K. Nakamura
拟南芥的TONSOKU(TSK)/MGOUN3/BRUSHY1编码一个与基因组维持有关的亮氨酸-甘氨酸-天冬氨酸(LGN)结构域蛋白，而TSK基因缺失的突变体表现为茎扁平，分生组织结构紊乱。我们从烟草BY-2细胞中鉴定出一个TSK的同源物(NtTSK)，它在LGN结构域和与AtTSK的富含亮氨酸的重复序列中都显示出高度的序列保守性。NtTSK基因在烟草BY-2细胞中表达于细胞周期的S期，细胞分裂同步化程度较高。与野生型相比，拟南芥tsk突变体含有较高比例的4C核细胞和表达细胞周期蛋白B1的细胞。这些结果表明，TSK在细胞周期中是必需的，TSK的缺陷导致细胞周期停滞在G2/M期。
TONSOKU(TSK)/MGOUN3/BRUSHY1 of Arabidopsis thaliana encodes a nuclear leucine-glycine-aspargine (LGN) domain protein implicated to be involved in genome maintenance, and mutants with defects in TSK show a fasciated stem with disorganized meristem structures. We identified a homolog of TSK from tobacco BY-2 cells (NtTSK), which showed high sequence conservation both in the LGN domain and in leucine-rich repeats with AtTSK. The NtTSK gene was expressed during S phase of the cell cycle in tobacco BY-2 cells highly synchronized for cell division. The tsk mutants of Arabidopsis contained an increased proportion of cells with 4C nuclei and cells expressing cyclin B1 compared with the wild type. These results suggest that TSK is required during the cell cycle and defects of TSK cause the arrest of cell cycle progression at G2/M phase.