Chemical composition of mate tea leaves (Ilex paraguariensis):: A study of extraction methods
Chemical composition of mate tea leaves (Ilex paraguariensis):: A study of extraction methods
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DOI：
10.1002/jssc.200600024
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发表时间：
2006-12-01
影响因子：
3.1
通讯作者：
Caramao, Elina Bastos
中科院分区：
文献类型：
--
作者：
Jacques, Rosangela Assis;Freitas, Lisiane dos Santos;Caramao, Elina Bastos
本工作的目的是研究提取巴拉圭冬青叶通过三种提取技术：加压液体提取（PLE，也称为加速溶剂提取- ASE），浸渍，超声。从在Industria e Comercio de Erva-Mate Barao LTDA，Brazil在农艺控制下进行的实验中收集交配茶叶的样品。本研究中使用了6种极性逐渐增加的溶剂（正己烷、甲苯、二氯甲烷、乙酸乙酯、丙酮和甲醇）。通过GC与质谱仪检测器联用对浸提液进行化学分析。通过共进样经认证的标准品完成鉴别和定量。结果表明，关于提取方法，提取物的质量没有显著差异。另一方面，PLE技术被发现是更有效的咖啡因，植醇，棕榈酸，硬脂酸的提取。与浸渍和超声辅助提取方法相比，PLE的使用导致总提取时间、溶剂消耗量和样品操作显著减少。
The objective of this work was to investigate the extraction of Ilex paraguariensis leaves by means of three extraction techniques: pressurized liquid extraction (PLE, also called accelerated solvent extraction - ASE), maceration, and sonication. Samples of mate tea leaves were collected from an experiment conducted under agronomic control at Industria e Comercio de Erva-Mate Barao LTDA, Brazil. Six solvents with increasing polarities (n-hexane, toluene, dichloromethane, ethyl acetate, acetone, and methanol) were used in this investigation. Chemical analysis of the extracts was performed by GC coupled with a mass spectrometer detector. The identification and quantification were accomplished by coinjections of certified standards. The results showed that no significant differences in the qualities of the extracts were noticed regarding the extraction methods. On the other hand, the PLE technique was found to be more effective for the extractions of caffeine, phytol, palmitic, and stearic acid. The use of PLE led to a significant decrease in the total extraction time, amount of solvent consumption, and manipulation of samples compared to maceration and ultra sound-assisted extraction methods.