Molecular identification on the causative species jellyfish blooms in the northern South China Sea in 2019
Molecular identification on the causative species jellyfish blooms in the northern South China Sea in 2019
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DOI：
10.11978/2021071
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发表时间：
2022-03-01
期刊：
影响因子：
--
通讯作者：
Dong Zhijun
中科院分区：
文献类型：
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作者：
Du Chong;He Jun;Dong Zhijun
水母水华主要发生在渤海、黄海和东中国海，但在南中国海很少发生。2019年5月，我们在南海中国北部海口、文昌、茂名等多个海域进行了水母盛开的形态观察和分子鉴定。形态观察结果表明，海口和文昌的水母同属一种，生殖点下部伞状，梨形突起，口臂末端有细丝和鞭状附属物，与鞭毛水母高度相似。茂名地区的水母则不同，伞形较平，半球形，次生殖窝呈乳白色突起，口臂无细丝，与南方猫头虫相似。根据线粒体核糖体大亚基(16S RRNA)基因序列比较，海口和文昌水母与东太平洋鞭毛水母的同源性分别为97.5%和97.7%，茂名水母与马六甲海峡的同源性为93.5%。根据线粒体COI基因和16S rRNA基因构建的系统发育树表明，海口和文昌的水母是相同的，与鞭毛虫同属一支；茂名的水母与乡土水母相近。因此，结合形态观察和分子分析，我们认为鞭毛虫是海口和文昌的水母，而卡特罗丝虫是海口和文昌的水母。在茂名。
Jellyfish blooms mostly occur in the Bohai Sea and Yellow Sea as well as in the East China Sea, but rarely in the South China Sea. We conducted morphological observations and molecular identification of blooming jellyfish in many waters of the northern South China Sea, including Haikou, Wenchang and Maoming in May 2019. Morphological results showed that the jellyfish in Haikou and Wenchang were the same species with hemispherical umbrella, pear-shaped protrusion in the lower reproductive point, and the oral arms are covered with filaments and a whip appendage at the end, which are highly similar to the Acromitus flagellatus. The jellyfish in Maoming were different: the umbrella was relatively flat hemispherical, the subgenital pits were milky protrusions and the oral arms had no filaments, which were similar to Catostylus townsendi. Based on the comparison of mitochondrial ribosomal large subunit (16S rRNA) gene sequences, the blooming jellyfish of Haikou and Wenchang were 97.5% and 97.7% similar to A. flagellatus of the eastern Pacific Ocean, and jellyfish of Maoming was 93.5% similar to C. townsendi from the Strait of Malacca. The phylogenetic tree constructed based on mitochondrial COI gene and 16S rRNA gene suggested that the jellyfish of Haikou and Wenchang were the same and belong to the same branch with A. flagellates; the jellyfish of Maoming were close to C. townsendi. Therefore, combined with morphological observations and molecular analyses, we considered Acromitus flagellatus to be the jellyfish in Haikou and Wenchang, and Catostylus sp. in Maoming.