Variable expression of Ia antigens in human endometrium and in chronic endometritis.
Variable expression of Ia antigens in human endometrium and in chronic endometritis.
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Ia 抗原在人子宫内膜和慢性子宫内膜炎中的可变表达。
DOI：
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发表时间：
1986
影响因子：
3.5
通讯作者：
M. Gerber
中科院分区：
文献类型：
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作者：
S. Tabibzadeh;A. Bettica;M. Gerber
最近的研究表明，Ia抗原可能在上皮细胞中表达，并且它们的表达可能受激素控制。因此，这些抗原的分布进行了研究，在冷冻切片的37个人的Ia与两个单克隆抗体（单克隆抗体）的单态性决定簇Ia抗原使用抗生物素蛋白-生物素复合物（ABC）的方法。5例早期增殖型，9例中期增殖型，3例晚期增殖型，12例分泌型乳腺癌。还使用了2例妊娠期输卵管炎和6例慢性输卵管炎患者。每个病例的四个连续切片进行Ia、OKT 8、Leu-3a和B1抗原染色。在整个周期中，子宫内膜间质中的内皮细胞、许多淋巴细胞和各种单核巨噬细胞呈Ia阳性。此外，Ia抗原定位于正常子宫内膜上皮。然而，Ia表达的强度和模式在周期的不同阶段有所不同。Ia抗原在增殖早期的子宫内膜腺体和表面上皮中染色较弱，在增殖中期和增殖晚期的基底层的表面上皮和腺细胞中染色较强，在功能层中染色较弱。Ia抗原在上皮细胞的表达是缺席或局灶性的分泌期和妊娠期子宫内膜。在整个周期和妊娠期子宫内膜中，与淋巴细胞聚集体密切相关的腺细胞呈Ia阳性。在慢性盆腔炎中，Ia阳性基质淋巴样细胞数量的增加与上皮中Ia抗原的强烈显示有关。研究结果表明，除了内皮细胞和淋巴细胞，Ia抗原表达在子宫内膜腺上皮细胞和表面上皮细胞。这种表达可能受到子宫内膜中淋巴样细胞和激素的影响。
Recent studies suggest that Ia antigens may be expressed in epithelial cells and that their expression may be under hormonal control. Therefore, the distribution of these antigens was studied in frozen sections of 37 human endometria with two monoclonal antibodies (Mab) to monomorphic determinants of Ia antigens using an avidin-biotin-complex (ABC) method. Five early proliferative, 9 midproliferative, 3 late proliferative, and 12 secretory endometria were examined. Two gestational endometria and six endometria with chronic endometritis were also used. Four consecutive sections from each case were stained for Ia, OKT8, Leu-3a, and B1 antigens. Throughout the cycle, the endothelial cells, many lymphocytes, and various monocytic-macrophagic cells in endometrial stroma were Ia positive. Furthermore, Ia antigens were localized to the normal endometrial epithelium. The intensity and the pattern of Ia expression, however, varied in different phases of the cycle. Ia antigens were stained weakly in endometrial glands and surface epithelium in early proliferative phase, and strongly in surface epithelium and glandular cells of the basalis and to a lesser extent of the functionalis in midproliferative and late proliferative phases. The expression of Ia antigens in epithelium was absent or focal during the secretory phase and in gestational endometria. Throughout the cycle and in gestational endometria, glandular cells in intimate association with lymphocytic aggregates were Ia positive. In chronic endometritis, the increased number of Ia positive stromal lymphoid cells was associated with a strong display of Ia antigens in epithelium. The findings indicate that, in addition to endothelial and lymphoid cells, Ia antigens are expressed in endometrial glandular and surface epithelial cells. This expression may be influenced by lymphoid cells in endometrium and by hormones.