Chemoprevention of azoxymethane-induced rat aberrant crypt foci by dietary zerumbone isolated from Zingiber zerumbet
Chemoprevention of azoxymethane-induced rat aberrant crypt foci by dietary zerumbone isolated from Zingiber zerumbet
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DOI：
10.1016/s0024-3205(01)01277-2
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发表时间：
2001-09-07
期刊：
影响因子：
6.1
通讯作者：
Mori, H
中科院分区：
文献类型：
--
作者：
Tanaka, T;Shimizu, M;Mori, H
以雄性F344大鼠为模型，研究了饲喂生姜中的泽伦骨对偶氮甲烷(AOM)诱导的大鼠结肠迷走性隐窝病灶(ACF)形成的影响。同时检测AOM和/或Zumbone对结肠粘膜环氧合酶(COX)-2表达的影响。此外，我们通过计数结肠隐窝细胞核内银染的核仁组织者区域蛋白(AgNORs)来评价泽龙骨对隐窝细胞增殖活性的影响。为建立ACF大鼠模型，每周3次皮下注射AOM(15 mg/kg体重)。从第一次注射AOM前一周开始，喂饲含0.01%或0.05%Zumbone的实验饲料5周。在研究结束时(第5周)，AOM暴露产生84+/-13个ACF/只大鼠。在0.01%和45+/-18剂量下，泽伦骨组ACF：72+/-17(减少14%)和45+/-18(减少46%，p
The modifying effects of dietary feeding of zerumbone isolated from Zingiber zerumbet on the development of azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) were investigated in male F344 rats. Expression of cyclooxygenase (COX)-2 in colonic mucosa exposed to AOM and/or zerumbone was also assayed. In addition, we assessed the effects of zerumbone on cell proliferation activity of crypts by counting silver-stained nucleolar organizer regions protein (AgNORs) in colonic cryptal cell nuclei. To induce ACF rats were given three weekly subcutaneous injections of AOM (15 mg/kg body weight). They were also fed the experimental diet containing 0.01% or 0.05% zerumbone for 5 weeks, starting one week before the first dosing of AOM. AOM exposure produced 84 +/- 13 ACF/rat at the end of the study (week 5). Dietary administration of zerumbone caused reduction in the frequency of ACF: 72 +/- 17 (14% reduction) at a dose of 0.01% and 45 +/- 18 (46% reduction, p