Effects of oxidized low-density lipoprotein on leukocyte-endothelial interactions in the rat mesenteric microcirculation during pregnancy
Effects of oxidized low-density lipoprotein on leukocyte-endothelial interactions in the rat mesenteric microcirculation during pregnancy
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DOI：
10.1016/j.ajog.2003.12.035
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发表时间：
2004-07-01
影响因子：
9.8
通讯作者：
Ito, M
中科院分区：
文献类型：
--
作者：
Katayama, T;Tanaka-Shiraishi, AT;Ito, M
目的：越来越多的证据表明氧化型脂蛋白与动脉粥样硬化有关。用这些脂蛋白治疗内皮细胞可以刺激单核细胞结合和趋化因子的产生，从而导致炎症和内皮损伤和功能障碍。在先兆子痫中，循环中的低密度脂蛋白颗粒增加，这是容易被氧化。我们研究了与氧化低密度脂蛋白相关的白细胞与内皮的相互作用。研究设计：利用带视频图像的活体显微镜，我们检测了妊娠期间氧化低密度脂蛋白诱导的大鼠肠系膜小静脉中白细胞的行为和对荧光标记的氧化低密度脂蛋白的摄取。结果：给予氧化低密度脂蛋白显著降低了小静脉中白细胞的滚动速度，并增加了与内皮细胞黏附的白细胞的数量。这些相互作用在妊娠期间减弱，标记的氧化低密度脂蛋白对白细胞和内皮细胞的摄取也减少。结论：妊娠可能与抗氧化作用有关。(C)2004 Elsevier Inc.保留所有权利。
Objective: The accumulation of evidence implicates oxidized lipoproteins in atherosclerosis. Treatment of endothelial cells with these lipoproteins stimulates monocyte binding and the production of chemotactic factors that contribute to inflammation and endothelial injury and dysfunction. In preeclampsia, circulating low-density lipoprotein particles, which are susceptible to oxidation, are increased. We studied leukocyte-endothelial interactions that were related to oxidized lipoproteins in pregnant rats.Study design: We examined oxidized low-density lipoprotein-induced leukocyte behavior and uptake of fluorescent-labeled oxidized low-density lipoprotein in rat mesenteric venules during pregnancy, with the use of intravital microscopy with a video imager.Results: The administration of oxidized low-density lipoprotein significantly reduced rolling velocities of leukocytes in venules and increased the numbers of leukocytes that adhered to endothelium in both nonpregnant and pregnant rats. These interactions were attenuated in pregnancy, when uptake of labeled oxidized low-density lipoprotein into leukocytes and endothelial cells also was decreased.Conclusion: Pregnancy may be associated with antioxidant effects. (C) 2004 Elsevier Inc. All rights reserved.