In Vivo Mapping of Hydrogen Peroxide and Oxidized Glutathione Reveals Chemical and Regional Specificity of Redox Homeostasis
In Vivo Mapping of Hydrogen Peroxide and Oxidized Glutathione Reveals Chemical and Regional Specificity of Redox Homeostasis
复制标题
DOI：
10.1016/j.cmet.2011.10.010
复制
发表时间：
2011-12-07
期刊：
影响因子：
29
通讯作者：
Dick, Tobias P.
中科院分区：
文献类型：
--
作者：
Albrecht, Simone C.;Barata, Ana Gomes;Dick, Tobias P.
谷胱甘肽氧化还原对（GSH/GSSG）和过氧化氢（H2 O2）是氧化还原稳态和氧化还原信号的核心，但它们在生物体内的分布很难测量。使用基因编码的氧化还原探针在果蝇中，我们建立定量的谷胱甘肽氧化还原电位（E-GSH）和H2 O2在整个动物的发展和衰老过程中定义的亚细胞室（胞质溶胶和线粒体）在体内映射。一种化学策略，以捕获在标本解剖和固定过程中的转基因生物传感器的体内氧化还原状态的荧光氧化还原成像的范围扩大到包括深部组织的成年苍蝇。我们发现，发展A和老化与氧化还原的变化，是明显的氧化还原对，亚细胞室，和组织特异性。中肠肠上皮细胞被确定为年龄依赖性胞质H2 O2积累的主要场所。更长的寿命与肠道中氧化剂形成的增加有关，而不是减少。
The glutathione redox couple (GSH/GSSG) and hydrogen peroxide (H2O2) are central to redox homeostasis and redox signaling, yet their distribution within an organism is difficult to measure. Using genetically encoded redox probes in Drosophila, we establish quantitative in vivo mapping of the glutathione redox potential (E-GSH) and H2O2 in defined subcellular compartments (cytosol and mitochondria) across the whole animal during development and aging. A chemical strategy to trap the in vivo redox state of the transgenic biosensor during specimen dissection and fixation expands the scope of fluorescence redox imaging to include the deep tissues of the adult fly. We find that development A and aging are associated with redox changes that are distinctly redox couple-, subcellular compartment-, and tissue-specific. Midgut enterocytes are identified as prominent sites of age-dependent cytosolic H2O2 accumulation. A longer life span correlated with increased formation of oxidants in the gut, rather than a decrease.