Kinetic measurements of gas exchange in the intact pulmonary microcirculation.
Kinetic measurements of gas exchange in the intact pulmonary microcirculation.
复制标题
完整肺微循环中气体交换的动力学测量。
DOI：
10.1152/jappl.1991.71.6.2536
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Klocke,RA
中科院分区：
文献类型：
--
作者：
Klocke,RA
在体积描记器内包含的隔离灌注肺制备物中监测气体交换的动力学。用缓冲液灌注肺，直到将2.0 ml反应物推注注入灌注系统中，才进行气体交换。随后的气体交换产生与交换气体的相应体积相关的压力瞬变。观察到的体积变化率是两个独立过程的结果：1）通过毛细血管床转运期间的气体交换率和2）注射点和毛细血管床之间血管通过时间的分布。后者通过含有溶解的惰性气体的对照注射来评估，所述惰性气体在丸剂进入毛细血管床时在肺泡中释放。对实验曲线的分析可以把这两个过程分开。交换动力学模型表明，该方法具有测量生理条件下微循环中气体交换过程中发生的动力学事件的能力。
The kinetics of gas exchange are monitored in an isolated perfused lung preparation contained within a plethysmograph. The lungs are perfused with buffer, and there is no gas exchange until a 2.0-ml bolus of reactant is injected into the perfusion system. Subsequent gas exchange produces a pressure transient that is related to the corresponding volume of exchanged gas. The observed rate of volume change is the result of two separate processes: 1) the rate of gas exchange during transit through the capillary bed and 2) the distribution of vascular transit times between the point of injection and the capillary bed. The latter is assessed by a control injection containing a dissolved inert gas that is liberated in the alveoli as the bolus enters the capillary bed. Analysis of the experimental curves permits the separation of these two processes. A model of exchange kinetics indicates that this method has the capability of measuring kinetic events occurring during gas exchange in the microcirculation under physiological conditions.