Kunitz trypsin inhibitor polymorphism in the Korean wild soybean (Glycine soja Sieb. & Zucc.)
Kunitz trypsin inhibitor polymorphism in the Korean wild soybean (Glycine soja Sieb. & Zucc.)
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DOI：
10.1111/pbr.12058
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发表时间：
2013-06
期刊：
影响因子：
2
通讯作者：
P. Krishnamurthy;Ram J. Singh;C. Tsukamoto;J. Park;Jeong Dong Lee;G. Chung
中科院分区：
文献类型：
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作者：
P. Krishnamurthy;Ram J. Singh;C. Tsukamoto;J. Park;Jeong Dong Lee;G. Chung
对1368份野生大豆材料的库尼茨胰酶抑制物(KTI)的研究&Zucc.)的等位基因多样性和地理分布。检测到五种可电泳性区分的KTI形式：三种是常见的(Tia、Tib和Tia/Tib)，两种以前没有报道(Tibi7-1和Tibi5)。Tia等位基因占优势(93.49%)。等位基因Tib、Tibi7-1和Tibi5的频率分别为3.47%、0.55%和0.11%。检测到Tia/Tib杂合型，频率为2.26%。Tibi7-1的核苷酸序列与Tib衍生的变异等位基因Tif的核苷酸序列相同，但有3个核苷酸不同：AG位于+244位，AC位于+286位，GC位于+601位。后两个等位基因与Tia相似，提示Tibi7-1是介于Tia和Tib之间的中间等位基因。Tibi5的基因与日本的中间等位基因Tibi5有100%的相似性。这项研究表明，韩国野生大豆是新的KTI等位基因的丰富来源，以前没有报道过。
Kunitz trypsin inhibitor (KTi) in 1368 accessions of wild soybean (Glycine soja Sieb. & Zucc.), collected from three regions of Korea, was examined for allelic diversity and geographical distribution. Five electrophoretically distinguishable KTi forms were detected: three were common (Tia, Tib and Tia/Tib) and two were previously unreported (Tibi7-1 and Tibi5). The Tia allele was predominant (93.49%). Alleles Tib, Tibi7-1 and Tibi5 were detected with the frequencies of 3.47, 0.55 and 0.11%, respectively. The heterozygous form (Tia/Tib) was detected with the frequency of 2.26%. The nucleotide sequence of Tibi7-1 was identical to that of the Tib-derived variant allele Tif, with the exception of three nucleotides: AG at position +244, AC at position +286 and GC at position +601. The latter two were similar to Tia, suggesting that Tibi7-1 is an intermediate allele between Tia and Tib. The gene for Tibi5 showed 100% similarity with the Japanese intermediate allele Tibi5. This study demonstrates that Korean wild soybeans are remarkably rich source of new KTi alleles not reported before.