Age-related changes in Kv4/Shal and Kv1/Shaker expression in Drosophila and a role for reactive oxygen species.
Age-related changes in Kv4/Shal and Kv1/Shaker expression in Drosophila and a role for reactive oxygen species.
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DOI：
10.1371/journal.pone.0261087
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Tsunoda S
中科院分区：
文献类型：
--
作者：
Vallejos MJ;Eadaim A;Hahm ET;Tsunoda S
Age-related changes in ion channel expression are likely to affect neuronal signaling. Here, we examine how age affects Kv4/Shal and Kv1/Shaker K+ channel protein levels in Drosophila. We show that Kv4/Shal protein levels decline sharply from 3 days to 10 days, then more gradually from 10 to 40 days after eclosion. In contrast, Kv1/Shaker protein exhibits a transient increase at 10 days that then stabilizes and eventually declines at 40 days. We present data that begin to show a relationship between reactive oxygen species (ROS), Kv4/Shal, and locomotor performance. We show that Kv4/Shal levels are negatively affected by ROS, and that over-expression of Catalase or RNAi knock-down of the ROS-generating enzyme, Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Oxidase (NOX), can attenuate the loss of Kv4/Shal protein. Finally, we compare levels of Kv4.2 and Kv4.3 in the hippocampus, olfactory bulb, cerebellum, and motor cortex of mice aged 6 weeks and 1 year. While there was no global decline in Kv4.2/4.3 that parallels what we report in Drosophila, we did find that Kv4.2/4.3 are differentially affected in various brain regions; this survey of changes may help inform mammalian studies that examine neuronal function with age.
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影响因子：
3.7
作者：
Davis KE;Fox S;Gigg J
通讯作者：
Gigg J
影响因子：
16.2
作者：
Bergquist, Sharon;Dickman, Dion K.;Davis, Graeme W.
通讯作者：
Davis, Graeme W.
影响因子：
8.8
作者：
Eadaim A;Hahm ET;Justice ED;Tsunoda S
通讯作者：
Tsunoda S
DOI：
10.1073/pnas.1206171109
发表时间：
2012-05-29
影响因子：
11.1
作者：
Busche, Marc Aurel;Chen, Xiaowei;Konnerth, Arthur
通讯作者：
Konnerth, Arthur
影响因子：
5.3
作者：
Cotella, Diego;Hernandez-Enriquez, Berenice;Sesti, Federico
通讯作者：
Sesti, Federico