MYCOPLASMA PNEUMONIAE IS A POLYCLONAL B-CELL ACTIVATOR
MYCOPLASMA PNEUMONIAE IS A POLYCLONAL B-CELL ACTIVATOR
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DOI：
10.1038/261238a0
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发表时间：
1976-01-01
期刊：
影响因子：
64.8
通讯作者：
GRONOWICZ, E
中科院分区：
文献类型：
--
作者：
BIBERFELD, G;GRONOWICZ, E
肺炎支原体（MP）是人类下呼吸道疾病的重要病原体1。当在MP抗原存在下培养时，来自经历MP感染的受试者的淋巴细胞显示出高度刺激2，3。但高浓度的MP抗原也在一定程度上刺激来自先前没有MP感染的血清学证据的个体的淋巴细胞，这表明这些受试者过去感染过MP，或者MP也具有非抗原特异性的促有丝分裂效应2。已知有几种促分裂原：例如，植物凝集素植物血凝素（PHA）和伴刀豆球蛋白A对T淋巴细胞4和某些细菌产物如大肠杆菌的脂多糖（LPS）具有促有丝分裂作用。大肠杆菌4和结核菌素的纯化蛋白衍生物（PPD）对小鼠B细胞5具有促有丝分裂作用，并诱导小鼠脾细胞4合成多克隆抗体。这些物质被称为多克隆B细胞激活剂（PBA）（参考文献6）。我们现在报告，MP是小鼠和豚鼠淋巴细胞有丝分裂原，并诱导小鼠脾细胞多克隆抗体的形成。
MYCOPLASMA PNEUMONIAE (MP) is an important causative agent of lower respiratory tract illness in man1. Lymphocytes from subjects who have experienced MP infection show a high degree of stimulation when cultured in the presence of MP antigen2,3. But high concentrations of MP antigen also stimulate, to some extent, lymphocytes from individuals without serological evidence of previous MP infection, suggesting that these subjects have either been infected with MP in the past or that MP also has a non-antigen-specific, mitogenic effect2. Several mitogens are known : the plant lectins phytohaemagglutinin (PHA) and concanavalin A, for example, have a mitogenic effect on T lymphocytes4, and certain bacterial products, such as lipopolysaccharide (LPS) ofE. coli4and a purified protein derivative of tuberculin (PPD) are mitogenic for mouse B cells5and induce polyclonal antibody synthesis in mouse spleen cells4. These substances are called polyclonal B-cell activators (PBA) (ref. 6). We now report that MP is mitogenic for mouse and guinea pig lymphocytes and induces polyclonal antibody formation in mouse spleen cells.