The future of NMR-based metabolomics.
The future of NMR-based metabolomics.
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DOI：
10.1016/j.copbio.2016.08.001
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发表时间：
2017-02
影响因子：
7.7
通讯作者：
Wishart DS
中科院分区：
文献类型：
--
作者：
Markley JL;Brüschweiler R;Edison AS;Eghbalnia HR;Powers R;Raftery D;Wishart DS
代谢组学的两种主要分析方法是质谱仪(MS)和核磁共振(核磁共振)谱。尽管目前在解析化合物的数量方面与MS相比相形见绌，但核磁共振波谱具有其自身和与MS结合的优势，核磁共振数据在很大的动态范围内具有高度的重复性和定量，在确定未知结构方面是无与伦比的。核磁共振擅长使用同位素标记来追踪代谢途径和通量。此外，核磁共振是非破坏性的，可以在体内使用。核磁共振结果在将体外研究结果转化为体内临床应用方面有着良好的记录。
The two leading analytical approaches to metabolomics are mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. Although currently overshadowed by MS in terms of numbers of compounds resolved, NMR spectroscopy offers advantages both on its own and coupled with MS. NMR data are highly reproducible and quantitative over a wide dynamic range and are unmatched for determining structures of unknowns. NMR is adept at tracing metabolic pathways and fluxes using isotope labels. Moreover, NMR is non-destructive and can be utilized in vivo. NMR results have a proven track record of translating in vitro findings to in vivo clinical applications.