Influence of iodoform on antimicrobial potential of calcium hydroxide.
Influence of iodoform on antimicrobial potential of calcium hydroxide.
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DOI：
10.1590/s1678-77572006000100007
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发表时间：
2006-01
期刊：
影响因子：
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通讯作者：
Pécora JD
中科院分区：
文献类型：
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作者：
Estrela C;Estrela CR;Hollanda AC;Decurcio Dde A;Pécora JD
本研究的目的是验证碘仿对氢氧化钙抗菌潜力的影响。S.金黄色葡萄球菌E.粪球菌、铜绿假单胞菌、B. subtilis、C.白色念珠菌是生物指示剂。供试物质为：氢氧化钙+生理盐水;氢氧化钙+碘仿+生理盐水;碘仿+生理盐水。对于琼脂扩散试验，用微生物悬液接种18个含20 ml BHI琼脂的培养皿。制作了54个空腔并填充了测试物质。然后测量微生物抑制的直径。在直接暴露试验中，将162个#50无菌吸水纸点浸入实验悬浮液中5 min，并用糊剂覆盖。以24、48和72小时的间隔，将纸尖浸入10 ml Letheen肉汤中，然后在37°C下孵育48小时。通过培养基的浊度评价微生物生长。将从Letheen肉汤获得的0.1 ml接种物转移到7 ml BHI中，并在37°C下孵育48小时。通过培养基的浊度再次评价细菌生长。氢氧化钙与生理盐水或碘仿加生理盐水结合在两种实验方法中均显示出抗菌效果。碘仿糊剂对所有生物微生物的琼脂扩散试验和对B的直接暴露试验均显示抗菌无效。枯草杆菌和混合物。
The purpose of this research was to verify the influence of Iodoform on antimicrobial potential of calcium hydroxide. S. aureus, E. faecalis, P. aeruginosa, B. subtilis, C. albicans were the biological indicators. The substances tested were: calcium hydroxide + saline; calcium hydroxide + Iodoform + saline; Iodoform + saline. For the agar diffusion test, 18 Petri plates with 20 ml of BHI agar were inoculated with the microbial suspensions. Fifty-four cavities were made and filled with the substances tested. The diameters of microbial inhibition were then measured. In direct exposure test, 162 #50 sterile absorbent paper points were immersed in the experimental suspensions for 5 min, and covered with the pastes. At intervals of 24, 48 and 72 hours, the paper points were immersed in 10 ml of Letheen Broth, followed by incubation at 37°°C for 48h. Microbial growth was evaluated by turbidity of the culture medium. A 0.1 ml inoculum obtained from the Letheen Broth was transferred to 7 ml of BHI, and incubated at 37°°C for 48h. Bacterial growth was again evaluated by turbidity of the culture medium. The calcium hydroxide associated with the saline or the iodoform plus saline showed antimicrobial effectiveness in both experimental methods. The iodoform paste presented antimicrobial ineffectiveness for the agar diffusion test on all biological microorganisms and for the direct exposure test on B. subtilis and on the mixture.