Orexinergic fibers are in contact with Kölliker-Fuse nucleus neurons projecting to the respiration-related nuclei in the medulla oblongata and spinal cord of the rat.
Orexinergic fibers are in contact with Kölliker-Fuse nucleus neurons projecting to the respiration-related nuclei in the medulla oblongata and spinal cord of the rat.
复制标题
食欲素能纤维与 K 接触
DOI：
10.1016/j.brainres.2016.08.020
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发表时间：
2016
期刊：
影响因子：
2.9
通讯作者：
Yukihiko Yasui
中科院分区：
文献类型：
--
作者：
Shigefumi Yokota;Tatsuro Oka;Hirohiko Asano;Yukihiko Yasui
呼吸变化依赖于警觉状态的神经通路仍然不稳定。本研究采用逆行追踪和免疫组织化学相结合的方法，研究了Köliker-Vouchnucleus（KFN）神经元向吻腹侧呼吸群（rVRG）、膈核（PhN）和舌下神经核（HGN）发出的食欲素能神经支配。将霍乱毒素B亚单位（CTB）注入KFN后，在下丘脑ORX野的穹窿周围区和内侧区以及外侧区均发现CTb标记的神经元，这些神经元对食欲素（orexin，ORX）也呈免疫反应。在rVRG、PhN或HGN内注入荧光金（FG）后，我们发现在KFN内ORX阳性轴突终末和FG阳性神经元的分布是重叠的。在KFN的神经元内，这些终末和神经元之间形成不对称的突触接触。我们进一步证明，许多神经元标记FG注射到rVRG，PhN，或HGN的ORX受体2的免疫反应。目前的数据表明，rVRG-，PhN-和HGN-投射KFN神经元的兴奋性影响下的ORX能神经元的状态依赖性调节呼吸。
The neural pathways underlying the respiratory variation dependent on vigilance states remain unsettled. In the present study, we examined the orexinergic innervation of Kölliker-Fuse nucleus (KFN) neurons sending their axons to the rostral ventral respiratory group (rVRG) and phrenic nucleus (PhN) as well as to the hypoglossal nucleus (HGN) by using a combined retrograde tracing and immunohistochemistry. After injection of cholera toxin B subunit (CTb) into the KFN, CTb-labeled neurons that are also immunoreactive for orexin (ORX) were found prominently in the perifornical and medial regions and additionally in the lateral region of the hypothalamic ORX field. After injection of fluorogold (FG) into the rVRG, PhN or HGN, we found an overlapping distribution of ORX-immunoreactive axon terminals and FG-labeled neurons in the KFN. Within the neuropil of the KFN, asymmetrical synaptic contacts were made between these terminals and neurons. We further demonstrated that many neurons labeled with FG injected into the rVRG, PhN, or HGN are immunoreactive for ORX receptor 2. Present data suggest that rVRG-, PhN- and HGN-projecting KFN neurons may be under the excitatory influence of the ORXergic neurons for the state-dependent regulation of respiration.