Unidirectional transfer of broad host-range plasmid R1162 during conjugative mobilization. Evidence for genetically distinct events at oriT.
Unidirectional transfer of broad host-range plasmid R1162 during conjugative mobilization. Evidence for genetically distinct events at oriT.
复制标题
接合动员过程中广泛宿主范围质粒 R1162 的单向转移。
DOI：
10.1016/0022-2836(89)90513-5
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发表时间：
1989
影响因子：
5.6
通讯作者：
Meyer,RJ
中科院分区：
文献类型：
--
作者：
Kim,K;Meyer,RJ
将含有接合动员基因（Mob）和转移起始基因（oriT）的R1162 DNA片段整合到大肠杆菌染色体中。细菌基因在整合片段的接合动员期间通过自传递质粒载体以极性方式转移。转移的方向，连同突变的衍生物oforiT的性质，表明初始切割oforiT，和随后的再连接转移后，需要不同的机制，可以区分遗传。
A segment of R1162 DNA containing genes for conjugative mobilization (Mob) and the origin of transfer (oriT) was integrated into theEscherichia colichromosome. Bacterial genes were transferred in a polar fashion during conjugative mobilization of the integrated segment by a self-transmissible plasmid vector. The direction of transfer, together with the properties of mutated derivatives oforiT, indicate that initial cleavage oforiT, and subsequent religation after transfer, entail different mechanisms that can be distinguished genetically.