MMP-10/stromelysin-2 promotes invasion of head and neck cancer.
MMP-10/stromelysin-2 promotes invasion of head and neck cancer.
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DOI：
10.1371/journal.pone.0025438
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Takata T
中科院分区：
文献类型：
--
作者：
Deraz EM;Kudo Y;Yoshida M;Obayashi M;Tsunematsu T;Tani H;Siriwardena SB;Keikhaee MR;Qi G;Iizuka S;Ogawa I;Campisi G;Lo Muzio L;Abiko Y;Kikuchi A;Takata T
Periostin, IFN-induced transmembrane protein 1 (IFITM1) and Wingless-type MMTV integration site family, member 5B (Wnt-5b) were previously identified as the invasion promoted genes of head and neck squamous cell carcinoma (HNSCC) by comparing the gene expression profiles between parent and a highly invasive clone. We have previously reported that Periostin and IFITM1 promoted the invasion of HNSCC cells. Here we demonstrated that Wnt-5b overexpression promoted the invasion of HNSCC cells. Moreover, stromelysin-2 (matrix metalloproteinase-10; MMP-10) was identified as a common up-regulated gene among Periostin, IFITM1 and Wnt-5b overexpressing HNSCC cells by using microarray data sets. In this study, we investigated the roles of MMP-10 in the invasion of HNSCC. We examined the expression of MMP-10 in HNSCC cases by immunohistochemistry. High expression of MMP-10 was frequently observed and was significantly correlated with the invasiveness and metastasis in HNSCC cases. Next, we examined the roles of MMP-10 in the invasion of HNSCC cells in vitro. Ectopic overexpression of MMP-10 promoted the invasion of HNSCC cells, and knockdown of MMP-10 suppressed the invasion of HNSCC cells. Moreover, MMP-10 knockdown suppressed Periostin and Wnt-5b-promoted invasion. Interestingly, MMP-10 overexpression induced the decreased p38 activity and MMP-10 knockdown induced the increased p38 activity. In addition, treatment with a p38 inhibitor SB203580 in HNSCC cells inhibited the invasion. These results suggest that MMP-10 plays an important role in the invasion and metastasis of HNSCC, and that invasion driven by MMP-10 is partially associated with p38 MAPK inhibition. We suggest that MMP-10 can be used as a marker for prediction of metastasis in HNSCC.
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影响因子：
11.5
作者：
Hatano, Hiroko;Kudo, Yasusei;Takata, Takashi
通讯作者：
Takata, Takashi
影响因子：
1.4
作者：
Kawata, R;Shimada, T;Murakami, Y
通讯作者：
Murakami, Y
影响因子：
8
作者：
Chen, R-S;Song, Y-M;Li, W.
通讯作者：
Li, W.
影响因子：
4.8
作者：
Dissanayake, Samudra K.;Wade, Michael;Weeraratna, Ashani T.
通讯作者：
Weeraratna, Ashani T.
影响因子：
15.9
作者：
Brakebusch, C;Bouvard, D;Fässler, R
通讯作者：
Fässler, R