Identification and molecular analysis of the gene encoding Rickettsia typhi hemolysin.
Identification and molecular analysis of the gene encoding Rickettsia typhi hemolysin.
复制标题
伤寒立克次体溶血素编码基因的鉴定和分子分析。
DOI：
10.1128/iai.67.11.6104-6108.1999
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发表时间：
1999
影响因子：
3.1
通讯作者：
Azad,AF
中科院分区：
文献类型：
--
作者：
Radulovic,S;Troyer,JM;Beier,MS;Lau,AO;Azad,AF
Rickettsia typhi, the causative agent of murine typhus, grows directly within the host cell cytoplasm, accumulating a large number of progeny, and eventually lyses the cells. Typhus group rickettsiae (R. typhiandR. prowazekii) adhere to and lyse human and sheep erythrocytes. However, the molecular mechanism underlying erythrocyte lysis byR. typhihas not been defined. Here we describe the cloning and nucleotide sequence analysis of the gene (tlyC) encoding a hemolysin fromR. typhi. DNA sequence analysis ofR. typhi tlyCrevealed an open reading frame of 912 bp, which encodes a protein of 304 amino acids with a predicted molecular mass of 38 kDa. To associate theR. typhi tlyCgene product with hemolytic activity, we performed complementation studies with hemolysin-negativeProteus mirabilisWPM111 (a HpmA−mutant of BA6163) transformed withR. typhi tlyCorR. typhi GFPuv-tlyCconstructs. We demonstrated that the clonedtlyCgene conferred a hemolytic phenotype on an otherwise nonhemolytic mutant ofP. mirabilis. The availability of the clonedR. typhi tlyCwill permit further characterization and definition of its role in rickettsial virulence.
