Delivery of demineralized bone matrix powder using a thermogelling chitosan carrier.
Delivery of demineralized bone matrix powder using a thermogelling chitosan carrier.
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DOI：
10.1016/j.actbio.2011.10.030
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发表时间：
2012-02
影响因子：
9.7
通讯作者：
Meng Tian;Zhi Yang;K. Kuwahara;M. Nimni;C. Wan;B. Han
中科院分区：
文献类型：
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作者：
Meng Tian;Zhi Yang;K. Kuwahara;M. Nimni;C. Wan;B. Han
脱矿骨基质（DBM）粉末因其具有骨诱导性和骨传导性而广泛用于骨再生。然而，操作困难、其从移植部位迁移的趋势以及术后缺乏稳定性有时会限制该材料的临床应用。在这项工作中，使用热凝胶壳聚糖载体提供DBM粉末的可能性进行了评估。DBM-热胶凝腻子改善了处理，并在体温下在临床相关时间段内原位形成凝胶状复合物。所形成的复合材料的性能，包括形态，孔隙率，力学性能，平衡溶胀以及降解性，显着影响的比例DBM的热凝胶壳聚糖。体外研究表明，包埋在复合材料中的C2 C12细胞的碱性磷酸酶活性随培养时间的延长而稳定增加。体内研究表明，DBM-热凝胶化壳聚糖中DBM含量的增加在裸鼠模型中诱导异位骨形成。生长因子从DBM-热凝胶壳聚糖的扩散以及宿主-植入物相互作用进行了讨论。
Demineralized bone matrix (DBM) powder is widely used for bone regeneration due to its osteoinductivity and osteoconductivity. However, difficulties with handling, its tendency to migrate from graft sites, and lack of stability after surgery can sometimes limit the clinical utility of this material. In this work, the possibility of using a thermogelling chitosan carrier to deliver DBM powder was assessed. The DBM-thermogelling putty improved handling and formed a gel-like composite in situ at body temperature within a clinically relevant time period. The properties of the formed composite, including morphology, porosity, mechanical properties, equilibrium swelling as well as degradability, are significantly influenced by the ratio of DBM to thermogelling chitosan. The in vitro study showed that the alkaline phosphatase activity of C2C12 cells encapsulated in the composite was steadily increased with culture time. The in vivo study showed that increased DBM content in the DBM-thermogelling chitosan induced ectopic bone formation in a nude rat model. The diffusion of growth factor from the DBM-thermogelling chitosan as well as the host–implant interactions are discussed.