Quantitative analysis of thymosin beta-10 messenger RNA in thyroid carcinomas.
Quantitative analysis of thymosin beta-10 messenger RNA in thyroid carcinomas.
复制标题
甲状腺癌中胸腺素β-10信使RNA的定量分析。
DOI：
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发表时间：
2002
影响因子：
2.4
通讯作者：
N. Amino
中科院分区：
文献类型：
--
作者：
T. Takano;Y. Hasegawa;A. Miyauchi;F. Matsuzuka;H. Yoshida;K. Kuma;N. Amino
背景
在良性和恶性组织中差异表达的基因对于建立基于分子的癌症诊断是重要的。我们最近对甲状腺癌基因表达谱的研究显示，胸腺素β-10 mRNA的表达增加。
方法
为了证实这一点，我们测量了84甲状腺良性和恶性甲状腺组织，包括5个未分化癌，通过实时定量逆转录聚合酶链反应的胸腺素β-10 mRNA的表达水平。
结果
我们发现胸腺素β-10 mRNA在甲状腺癌中的表达增加，特别是在未分化癌中。胸腺素β-10 mRNA相对于甲状腺球蛋白mRNA在未分化癌中的表达水平显著高于分化癌。
结论
这些结果表明，有用的胸腺素β-10 mRNA的定量测量的分子为基础的诊断甲状腺未分化癌，但不是分化癌。
BACKGROUND
Genes that are differentially expressed in benign and malignant tissues are important for the establishment of molecular-based diagnosis of carcinomas. Our recent study on the gene expression profile of thyroid carcinomas revealed an increased expression of thymosin beta-10 mRNA.
METHODS
To confirm this, we measured the expression levels of thymosin beta-10 mRNA in 84 thyroid benign and malignant thyroid tissues, including five anaplastic carcinomas, by means of real-time quantitative reverse transcription-polymerase chain reaction.
RESULTS
We found an increased expression of thymosin beta-10 mRNA in thyroid carcinomas, especially in anaplastic carcinomas. Expression levels of thymosin beta-10 mRNA relative to thyroglobulin mRNA in anaplastic carcinomas were greatly increased compared with those in differentiated carcinomas.
CONCLUSION
These results suggest the usefulness of the quantitative measurement of thymosin beta-10 mRNA in molecular-based diagnosis of thyroid anaplastic carcinomas, but not of differentiated carcinomas.
影响因子：
4.4
作者：
H. Gondo;J. Kudo;J. W. White;C. Barr;P. Selvanayagam;G. Saunders
通讯作者：
H. Gondo;J. Kudo;J. W. White;C. Barr;P. Selvanayagam;G. Saunders